Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/22909
Title: Genetic profiles and antimicrobial resistance of Streptococcus pneumoniae non-PCV10 serotype isolates recovered from meningitis cases in Salvador, Brazil
Authors: Silva Junior, Jailton de Azevedo
Anjos, Eder Silva dos
Cordeiro, Soraia Machado
Santos, Milena Soares dos
Escobar, Eliane C
Lobo, Paulo Rocha
Carvalho, Maria da Gloria
Reis, Mitermayer Galvão dos
Reis, Joice Neves
Campos, Leila Carvalho
Affilliation: Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil
Universidade Federal da Bahia. Instituto Multidisciplinar em Saúde. Vitória da Conquista, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Respiratory Diseases Branch. Centers for Disease Control and Prevention. Atlanta, USA
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Abstract: In 2010, the 10-valent pneumococcal conjugate vaccine (PCV10) was introduced into the Brazilian childhood vaccination programme. Concerns have been raised that non-vaccine serotypes could increase in prevalence and reduce the benefits of vaccination; therefore, we examined non-PCV10 isolates recovered from meningitis during pre- (January 2008-May 2010) and post-vaccine (June 2010-December 2012) periods. Surveillance for pneumococcal meningitis was established at the Reference Hospital of Infectious Diseases in Salvador, Brazil. Serotypes were determined by multiplex PCR and/or Quellung reaction. Antimicrobial susceptibility testing was conducted by E-test and broth microdilution. Genotyping used PFGE and multi-locus sequence typing. A total of 148 cases of meningitis were identified from January 2008 to December 2012, 77 (52 %) of which were due to non-PCV10 isolates, with 50 (52.1 %) from pre-vaccine and 27 (52 %) from post-vaccine periods. In the post-vaccine period, the non-PCV10 serotypes 12F (n=6; 22.2 %), 10A (n=3; 11.1 %), 15B (n=2; 7.4 %) and 18B (n=2; 7.4 %) were the most prevalent. Forty-three isolates (55.8 %) were non-susceptible to one or more antibiotics. Non-susceptibility to penicillin was observed among serotypes 19A (three isolates), 9N (one isolate) and 12F (one isolate). PFGE and multi-locus sequence typing results demonstrated a wide genetic diversity among the isolates. During the early period following PCV10 introduction, no obvious emergence of a particular serotype was evident among non-PCV10 strains. This study underscores the importance of monitoring any changes among non-PCV10 cases after the introduction of PCV10.
Keywords: Streptococcus pneumoniae
Pneumococcal Vaccines
Meningitis, Pneumococcal
Genetic Variation
Serotyping
Adolescent
Adult
Brazil
keywords: Streptococcus pneumoniae
Vacinas pneumocócicas
Meningite, pneumococo
Variação genética
Serotipagem
Adolescente
Adulto
Brasil
Issue Date: 2016
Publisher: Microbiology Society
Citation: SILVA JUNIOR, J. A. et al. Genetic profiles and antimicrobial resistance of Streptococcus pneumoniae non-PCV10 serotype isolates recovered from meningitis cases in Salvador, Brazil. Journal of Medical Microbiology, v. 65, p. 1164–1170, 2016.
DOI: 10.1099/jmm.0.000346
ISSN: 0022-2615
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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