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INCREASED SENSITIVITY OF NS1 ELISA BY HEAT DISSOCIATION IN ACUTE DENGUE 4 CASES
Buonora, Sibelle Nogueira et al. | Date Issued: 2017
Author
Buonora, Sibelle Nogueira
Santos, Flávia Barreto dos
Daumas, Regina Paiva
Passos, Sonia Regina Lambert
Silva, Manoela Heringer da
Lima, Monique Rocha de
Nogueira, Rita Maria Ribeiro
Affilliation
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivirus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Centro de Saúde Escola Germano Sinval Faria. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivirus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivirus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivirus. Rio de Janeiro, RJ. Brasil.
Abstract
Dengue is an acute febrile illness considered the major arboviral disease in terms of morbidity, mortality, economic impact and dissemination worldwide. Brazil accounts for the highest notification rate, with circulation of all four dengue serotypes. The NS1 antigen is a dengue highly conserved specific soluble glycoprotein essential for viral replication and viability that can be detected 0 to 18 days from the onset of fever (peak first 3 days). It induces a strong humoral response and is known as a complement-fixing antigen. Lower NS1 test sensitivity occurs in secondary dengue infections probably due to immune complex formation impairing antigen detection by ELISA.
Keywords in Portuguese
Ensaio de Imunoadsorção Enzimática
Precisão
Diagnóstico de Dengue
Dissociação do complexo imunitário
 
Keywords
NS1 ELISA
Accuracy
Dengue diagnosis
Immune complex dissociation
DENV-4
 
Publisher
BioMed Central
Citation
BUONORA, Sibelle Nogueira; et al. Increased sensitivity of NS1 ELISA by heat dissociation in acute dengue 4 cases. BMC Infectious Diseases, v.17:204, 5p, 2017.
DOI
10.1186/s12879-017-2306-z
ISSN
1471-2334