Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/25815
Title: RNA interference-mediated knockdown of CD49e (α5 integrin chain) in human thymic epithelial cells modulates the expression of multiple genes and decreases thymocyte adhesion
Authors: Lacerda, Leandra Linhares
Alves, Marcelo Ribeiro
Nogueira, Ana Cristina Martins de Almeida
Cruz, Daniella Areas Mendes da
Magalhães, Danielle Aparecida
Dardenne, Mireille
Passos, Geraldo Aleixo
Savino, Wilson
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Imunologia.. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ. Brasil.
Universidade de São Paulo. Escola de Medicina. Departamento de Genética. Grupo de Imunogenética Molecular. São Paulo, SP, Brasil.
Université Paris Descartes. Hôpital Necker. Centre National de la Recherche Scientifique. Paris, France.
Universidade de São Paulo. Escola de Medicina. Departamento de Genética. Grupo de Imunogenética Molecular. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ. Brasil.
Abstract: The thymus is a central lymphoid organ, in which bone marrow-derived T cell precursors undergo a complex process of maturation. Developing thymocytes interact with thymic microenvironment in a defined spatial order. A component of thymic microenvironment, the thymic epithelial cells, is crucial for the maturation of T-lymphocytes through cell-cell contact, cell matrix interactions and secretory of cytokines/chemokines. There is evidence that extracellular matrix molecules play a fundamental role in guiding differentiating thymocytes in both cortical and medullary regions of the thymic lobules. The interaction between the integrin α5β1 (CD49e/CD29; VLA-5) and fibronectin is relevant for thymocyte adhesion and migration within the thymic tissue. Our previous results have shown that adhesion of thymocytes to cultured TEC line is enhanced in the presence of fibronectin, and can be blocked with anti-VLA-5 antibody.
Keywords: gene regulation
heterocellular cell adhesion
gene regulation
thymocytes
thymic microenvironment
cd49E
keywords: Regulação gênica
adesão de células heterocelulares
Timócitos
microambiente tímico
células T intratímicas
Issue Date: 2010
Publisher: BioMed Central
Citation: LACERDA, Leandra Linhares et al. RNA interference-mediated knockdown of CD49e (a5 integrin chain) in human thymic epithelial cells modulates the expression of multiple genes and decreases thymocyte adhesion. BMC Genomics, v.1, Suppl. 5, p. :S2, 13 p, 2010.
Description: From 5th International Conference of the Brazilian Association for Bioinformatics and Computational Biology (X-meeting 2009) Angra Dos Reis, RJ, Brazil. 18-22 October 2009
DOI: 10.1186/1471-2164-11-S5-S2
ISSN: 1471-2164
Copyright: open access
Appears in Collections:INCQS - Artigos de Periódicos
CDTS - Artigos de Periódicos
IOC - Artigos de Periódicos

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