Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/26115
Title: Morphological studies of hepatic tissue of mice reinfected with dengue virus serotypes 1 or 2
Authors: Vieira, Debora Ferreira Barreto
Schatzmayr, Hermann Gonçalves
Takiya, Christina Maeda
Jácome, Fernanda Cunha
Silva, Manoel Enderson Vieira
Faria, Nieli Rodrigues da Costa
Nogueira, Rita Maria Ribeiro
Barth, Ortrud Monika
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Ciências Biológicas. Departamento de Histologia e Embriologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.
Abstract: Histopathological and ultrastructural aspects of liver of non-neuroadapted BALB/c mice reinfected by the intravenous route with dengue virus serotypes 1 and 2 were presented. The hepatic tissue was processed following the standard techniques for photonic and transmission electron microscopy. Morphological studies showed vacuolization of hepatocytes, inflammatory cells inside sinusoidal capillaries, enlargement of sinusoidal capillaries, foci of hemorrhage inside the interstitium, increase of surface density of reticular fibres, increase of numerical density of sinusoidal cells, decrease of surface density of hepatocytes, edema in the peri centrolobular vein space and presence of phyllopods and pseudopod-like extensions in endothelial cells. DENV particles, virus antigens and DENV RNA were observed in mosquito cells (C6/36) inoculated with sera of the animals 72 hours post-reinfection. The hepatic alterations observed in our experimental model were similar to those observed in human cases of dengue hemorrhagic fever. The present study shows that BALB/c mice reinfected with a heterologous serotype of DENV develop more severe lesions than those observed in mice in primary infection.
Keywords: dengue-2 virus
dengue-1 virus
BALB/c mice
liver
ultrastructure
histopathology
keywords: Dengue Virus-Tipo 2
Dengue Virus Tipo1
Fígado
Ultraestrutura
Histopatololgia
Camundongos Endogâmicos BALB C
Issue Date: 2010
Publisher: Sociedade Brasileira de Virologia
Citation: VIEIRA, Debora Ferreira Barreto; et al. Morphological studies of hepatic tissue of mice reinfected with dengue virus serotypes 1 or 2. Virus Review and Research, v.15, n.2, 27p, 2010.
ISSN: 1519-2563
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
debora_vieira_etal_IOC_2011.pdf1.53 MBAdobe PDFView/Open



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.