Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/27572
Title: Research about Listeria Sp., Salmonella Sp. And Others Contamination Indicators to Milk’s and Cheese’s Samples Sale in the South of Minas Gerais State
Authors: Carvalho, P. L. N.
Carvalhjo, P. R.
Hofer, Ernesto
Reis, C. M. F.
Veiga, S. M. O. M.
Affilliation: Universidade Federal de Alfenas. Alfenas, MG, Brasil.
Secretaria de Agricultura do Estado de São Paulo. Bauru, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ. Brasil.
Universidade Federal de Alfenas. Alfenas, MG, Brasil.
Abstract: A study was done in 112 milk samples and milk derivative samples, being 46 homemade cheese (QC) and industrialized cheese (QI) and 66 raw milk (LC) and pasteurized milk (LPC), in order to quantify mesophilics aerobics (AM), positive coagulase Staphylococci (SCP), MPN of totals coliforms (CT) and fecals (CF) and a research was made on E. coli, Salmonella sp., Pseudomonas aeruginosa and Listeria sp. For Listeria, the FDA official methodology was adopted including cold enrichment stage (45 days /4°C) and microaerofilic system. Count averages of AM for QI (4.88x109 CFU g-1) were significantly different (P <0.05) from QC. SCP counting showed 47.83% (QC) and 34.78% (QI) which exceeded default value. Averages (MPN g-1) for CT were 2.39x105 (QC) and 2.48x105 (QI); and for CF was 1.67x105 (QI). E. coli was detected in QC: 78.26% and in QI: 60.87%, Salmonella sp. was isolated in QI: 8.70% and P. aeruginosa was isolated in 100% of QC and QI. Listeria sp. was isolated before and after of cold enrichment and it was identified L. innocua 6a, 6b, 4ab and untypable. In the milk sampling the positivity for E. coli was 75.76% (LC), 39.39% (LPC) and 12.12% with isolation of Salmonella sp in LPC. It was concluded by the results that both milk and cheese were disqualified for consumption for offering potential risks to public health.
Keywords: Cold enrichment stage
Listeria
Salmonella
milk
cheese
microbiological quality
Brazil
keywords: Fase de enriquecimento frio
Listeria
Leite
Queijo
Samonela
qualidade microbiológica
Minas Gerais
Issue Date: 2009
Publisher: American-Eurasian Network for Scientific Information
Citation: CARVALHO, P. L. N. et al. Research about Listeria Sp., Salmonella Sp. And Others Contamination Indicators to Milk’s and Cheese’s Samples Sale in the South of Minas Gerais State. Australian Journal of Basic and Applied Sciences, v.3, n.4, p.4422-4431, 2009.
ISSN: 1991-8178
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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