Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/27577
Title: Multiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimens
Authors: Vasconcelos, Rafaela Moledo de
Almeida, Antonio Eugenio Castro Cardoso de
Hofer, Ernesto
Silva, Nicéa Magaly Matias da
Marin, Victor Augustus
Affilliation: Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde.. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Microbiologia. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Microbiologia. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ. Brasil.
Clínica Perinatal de Laranjeiras. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Microbiologia. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde.. Rio de Janeiro, RJ. Brasil.
Abstract: The genus Listeria is composed of six species of which Listeria monocytogenes is considered the single pathogenic species that causes listeriosis in humans. Of the 13 serovars of L. monocytogenes, 1/2a, 1/2b and 4b are responsible for the majority of clinical cases. The aim of this work was to detect L. monocytogenes in the cerebrospinal fluid sample of premature newborns and to characterize this sample using biotyping, serotyping and molecular typing. The results indicated the presence of L. monocytogenes in the clinical sample studied. Moreover, the isolate was identified as the 4b serovar that was characterized by the presence of a unique 691 bp band after analysis using the Multiplex-PCR technique. The results of repeated Multiplex-PCR and sequencing have indicated that the L. monocytogenes isolate was an atypical 4b serovar, which is the first time this finding has been reported.
Keywords: Listeria monocytogenes
listeriosis
Multiplex PCR
cerebrospinal fluid
keywords: Listeria monocytogenes
Reação em Cadeia da Polimerase Multiplex
Líquido Cefalorraquidiano
Listeriose
Issue Date: 2008
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Citation: VASCONCELLOS, Rafaela Moledo de; et al. Multiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimens. Mem Inst Oswaldo Cruz, v.103, n.8, p.836-838, Dec. 2008.
ISSN: 0074-0276
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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