Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/28924
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dc.contributor.authorCosta, Jimena Ferreira da
dc.contributor.authorFerrarini, Mariana Galvão
dc.contributor.authorNardelli, Sheila Cristina
dc.contributor.authorGoldenberg, Samuel
dc.contributor.authorÁvila, Andréa Rodrigues
dc.contributor.authorHoletz, Fabíola Barbieri
dc.date.accessioned2018-09-20T12:10:31Z
dc.date.available2018-09-20T12:10:31Z
dc.date.issued2018
dc.identifier.citationCOSTA, Jimena Ferreira da et al.Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v.113, n. 6, 2018.
dc.identifier.issn0074-0276
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/28924
dc.language.isopor
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.rightsopen access
dc.subject.otherTrypanosoma cruzi
dc.titleTrypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery
dc.typeArticle
dc.identifier.doi10.1590/0074-02760170531
dc.description.abstractenEukaryotic ribonucleoprotein (RNP) granules are important for the regulation of RNA fate. RNP granules exist in trypanosomatids; however, their roles in controlling gene expression are still not understood. XRNA is a component of granules in Trypanosoma brucei but has not been investigated in Trypanosoma cruzi. This study aimed to investigate the TcXRNA dynamic assembly and its interaction with RNP components under conditions that affect the mRNA availability. As methods, we used in vitro metacyclogenesis of T. cruzi to observe changes in RNP granules during the differentiation process. TcXRNA expression was analysed by Western blot and immunofluorescence. Colocalisation assays were performed to investigate the interaction of TcXRNA with other RNP components. It finded that TcXRNA is constantly present during metacyclogenesis and is localised in cytoplasmic granules. TcXRNA does not colocalise with TcDHH1 and TcCAF1 granules in the cytoplasm. However, TcXRNA granules colocalise with mRNP granules at the nuclear periphery when mRNA processing is inhibited. This way we have as main conclusions that, TcXRNA plays a role in mRNA metabolism as a component of mRNP granules whose assembly is dependent on mRNA availability. TcXRNA granules colocalise with distinct RNP granules at the nuclear periphery, suggesting that the perinuclear region is a regulatory compartment in T. cruzi mRNA metabolism.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
dc.subject.enRNA
dc.subject.enMessenger
dc.subject.enRibonucleoproteins
dc.subject.enGene Expression
dc.subject.esARN Mensajero
dc.subject.esExpresión Génica
dc.subject.decsRNA Mensageiro
dc.subject.decsRibonucleoproteínas
dc.subject.decsExpressão Gênica
dc.identifier.eissn1678-8060
Appears in Collections:PR - ICC - Artigos de Periódicos

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