Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/29947
Title: Trypanosoma cruzi: Effects of azadirachtin and ecdysone on the dynamic development in Rhodnius prolixus larvae
Authors: Cortez, M. R.
Provençano, A.
Silva, C. E.
Mello, C. B.
Zimmermann, L. T.
Schaub, G. A.
Garcia, E. S.
Azambuja, P.
Gonzalez, M. S.
Affilliation: Ministerio de Salud y Deportes Bolívia. Programa Nacional de Chagas. Unidad de Epidemiologia. La Paz, Bolivia.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Bioquímica de Insetos. Rio de Janeiro, RJ. Brasil.
Universidade Federal do Rio Grande do Sul. Instituto de Ciências Básicas da Saúde. Departamento de Microbiologia. Porto Alegre, RS, Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.
Ruhr- Universität Bochum. Parasitology and Zoology Group. Bochum, Germany.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Bioquímica de Insetos. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Entomologia Molecular. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Bioquímica de Insetos. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Entomologia Molecular. Brasil.
Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil / Instituto Nacional de Entomologia Molecular. Brasil.
Abstract: The effects of azadirachtin and ecdysone on the Trypanosoma cruzi population in the Rhodnius prolixus gut were investigated. T. cruzi were rarely found in the gut compartments of azadirachtin-treated larvae. High parasite numbers were observed in the stomach of the control and ecdysone groups until 10 days after treatment and in the small intestine and rectum until 25 days after treatment. High percentages of round forms developed in the stomachs of all groups, whereas azadirachtin blocked the development of protozoan intermediate forms. This effect was counteracted by ecdysone therapy. In the small intestine and rectum, epimastigotes predominated for all groups, but more of their intermediates developed in the control and ecdysone groups. Azadirachtin supported the development of round forms and their intermediates into trypomastigotes. In the rectum, trypomastigotes did not develop in the azadirachtin group and developed much later after ecdysone therapy. The parallel between the effects of azadirachtin and ecdysone on the host and parasite development is discussed on the basis of the present results because ecdysone appears to act directly or indirectly in determining the synchronic development of T. cruzi forms from round to epimastigotes, but not metacyclic trypomastigotes, in the invertebrate vector.
Keywords: Rhodnius prolixus
Trypanosoma cruzi
Developmental forms
Ecdysone
Azadirachtin
keywords: Rhodnius prolixus
Trypanosoma cruzi
Ecdisona
Formas de desenvolvimento
Azadiractina
Issue Date: 2012
Publisher: Elsevier
Citation: CORTEZ, M. R. et al. Trypanosoma cruzi: Effects of azadirachtin and ecdysone on the dynamic development in Rhodnius prolixus larvae. Experimental Parasitology. Experimental Parasitology v.131, p.363–371, May 2012
ISSN: 0014-4894
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
aprovençano_patriciazambuja_etal_IOC_2012.pdf617.96 kBAdobe PDF    Request a copy



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.