Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/30374
Title: Neolignans from plants in northeastern Brazil (Lauraceae) with activity against Trypanosoma cruzi
Authors: Cabral, M. M. O.
Barbosa Filho, J. M.
Maia, G. L. A.
Chaves, M. C. O.
Braga, M.V.
Souza, W. de
Soares, R. O. A.
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Diptera. Rio de Janeiro, RJ. Brasil.
Universidade Federal da Paraíba. Departamento de Tecnologia Farmacêutica. João Pessoa, PB, Brasil.
Universidade Federal da Paraíba. Departamento de Tecnologia Farmacêutica. João Pessoa, PB, Brasil.
Universidade Federal da Paraíba. Departamento de Tecnologia Farmacêutica. João Pessoa, PB, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ecoepidemiologia da Doença de Chagas. Rio de Janeiro, RJ. Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanosomatídeos. Rio de Janeiro, RJ. Brasil.
Abstract: Trypanosoma cruzi is the ethiological agent for Chagas disease in Latin America. This study aimed to test the trypanocidal effect of licarin A and burchellin isolated from plants in northeastern Brazil. These neolignans were tested on T. cruzi and on peritoneal macrophages, to evaluate drug toxicity. Epimastigote growth was inhibited in 45% with licarin A and 20% with burchellin with an IC(50)/96 h of 462.7 microM and 756 microM, respectively. Epimastigotes treated with licarin A presented swollen mitochondria and disorganized mitochondrial cristae, kDNA and Golgi complex. When treated with burchellin, they presented enormous autophagosomes and chromatin disorganization. Licarin A and burchellin were able to induce trypomastigote death with IC(50)/24 h of 960 microM and 520 microM, respectively. Although licarin A presented an IC(50) for trypomastigotes higher than for epimastigotes, both substances acted as therapeutic trypanocidal agents, because they were able to kill parasites without affecting macrophages. Due to our results, burchellin and licarin A need to be further analysed to observe if they may be used as alternative blood additive prophylaxis against Chagas disease, since it has been established that blood transfusion is an important mechanism in the transmission process.
Keywords: Nectandra glabrescens
Ocotea cymbarum
Trypanosoma cruzi
Licarin A
Burchellin
Neolignans
Chemotherapy
northeastern Brazil
keywords: Trypanosoma cruzi
Lignanas
Quimioterapia
Nordeste do Brazil
Plantas
Issue Date: 2010
Publisher: Elsevier
Citation: CABRAL, M. M. O. et al. Neolignans from plants in northeastern Brazil (Lauraceae) with activity against Trypanosoma cruzi. Experimental Parasitology, v.124, p.319–324, Nov. 2010.
DOI: 10.1016/j.exppara.2009.11.007
ISSN: 0014-4894
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
MMO_cabral_etal_IOC_2010.pdf387.58 kBAdobe PDF    Request a copy


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.