Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/30792
Title: The distribution of novel biomarkers in carcinoma-in-situ, microinvasive, and squamous cell carcinoma of the uterine cervix
Authors: Nicol, Alcina F.
Andrade, Cecilia Vianna de
Gomes Jr., Saint Clair
Brusadelli, Marion G.
Lodin, Hannah M.
Wells, Susanne I.
Nuovo, Gerard J.
Affilliation: Oswaldo Cruz Foundation. National Institute of Infectious Diseases Evandro Chagas. Rio de Janeiro, RJ, Brazil.
Oswaldo Cruz Foundation. National Institute of Health of Women, Children, and Adolescents, Fernandes Figueira. Rio de Janeiro, RJ, Brazil.
Oswaldo Cruz Foundation. National Institute of Health of Women, Children, and Adolescents, Fernandes Figueira. Rio de Janeiro, RJ, Brazil.
Cincinnati Children's Hospital Medical Center. Division of Oncology, Cancer and Blood Diseases Institute. Cincinnati, OH, USA.
Cincinnati Children's Hospital Medical Center. Division of Oncology, Cancer and Blood Diseases Institute. Cincinnati, OH, USA.
Cincinnati Children's Hospital Medical Center. Division of Oncology, Cancer and Blood Diseases Institute. Cincinnati, OH, USA.
The Ohio State University Comprehensive Cancer Center. Columbus, OH, USA / Phylogeny Inc, Powell. OH, USA.
Abstract: Importin-β, exportin-5, p16, Ki-67, Mcl1, PDL1, and cFLIP are each over-expressed in the majority of CIN 1 lesions. These biomarkers, plus HPV E6/E7 RNA, were analyzed in carcinoma-in-situ (CIS), microinvasive, and squamous cell carcinoma (SCC) of the uterine cervix and cervical carcinoma cell lines. Only p16 and Ki-67 continued to be over-expressed in CIS, with a concomitant marked increase in E6/E7 RNA. There was a highly significant increase in PDL1 expression and decrease in Ki-67 (each p < 0.001) in microinvasive cancer compared to CIS whereas p16 and E6/E7 remained stable. As the lesion progressed to SCC, p16 and E6/E7 RNA remained strongly overexpressed with a concomitant over expression of importin-β and Ki67. HPV positive Caski cells showed significant elevations of p16, importin-β, exportin-5 and PDL1 compared to the HPV negative cervical cancer cell line C33A, consistent with viral induction of these biomarkers. The data suggest that PDL1 may be a useful biomarker to differentiate CIS from microinvasive cancer and, thus, anti-PDL1 therapy may inhibit the progression of CIS to the invasive stage.
Keywords: Cervical cancer
Microinvasive
PDL-1
Biomarkers
Issue Date: 2019
Publisher: Elsevier
Citation: NICOL, Alcina F. et al. The distribution of novel biomarkers in carcinoma-in-situ, microinvasive, and squamous cell carcinoma of the uterine cervix. Annals of Diagnostic Pathology, v. 38, p. 115-122, 2019.
DOI: 10.1016/j.anndiagpath.2018.12.001
ISSN: 1092-9134
Copyright: restricted access
Appears in Collections:IFF - Artigos de Periódicos
INI - Artigos de Periódicos

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