Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31547
Title: Correlation between susceptibility of primary HIV-1 isolates to autologous and heterologous neutralizing antibodies
Authors: Bongertz, Vera
Costa, Catia I.
Veloso, Valdiléa G.
João Filho, Esaú C.
Galvão-Castro, Bernardo
Morgado, Mariza G.
Hospital Evandro Chagas AIDS Clinical Research Group
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.
Hospital dos Servidos do Estado do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Abstract: OBJECTIVE: To study the susceptibility of primary HIV-1 isolates towards autologous and heterologous neutralizing antibodies (NAb). DESIGN: Blood was collected and primary HIV-1 isolated from individuals residing in Rio de Janeiro, Brazil, in all phases of disease. METHODS: Primary HIV-1 isolates were incubated with autologous or heterologous plasma and neutralization of infection of freshly pre-stimulated normal human peripheral blood mononuclear cells was assayed in parallel to median infectious dose determinations in the absence of antibodies. Levels of HIV-1 p24 antigen were used for evaluation of viral neutralization. RESULTS: Autologous neutralization (75%) was observed for 13 (52%) out of 25 of the primary HIV-1 isolates, and 15 (71%) out of 21 isolates were susceptible to 75% heterologous neutralization by at least one-half of the heterologous plasma tested. Primary HIV-1 isolates susceptible to autologous NAb showed a higher susceptibility towards neutralization by heterologous NAb than isolates that could not be neutralized by the autologous plasma (P = 0.049). The susceptibility of the primary HIV-1 isolates towards neutralization by heterologous NAb was significantly higher for isolates derived from men (P = 0.001), and for isolates obtained from individuals infected through homo-/bisexual risk behaviour in comparison with those infected through heterosexual HIV-1 transmission (P = 0.03). CONCLUSIONS: Susceptibility of primary HIV-1 isolates to autologous and heterologous neutralization was significantly correlated, indicating that escape mutants may become resistant not only to autologous but also to heterologous NAb.
Keywords: Primary HIV-1 isolates
Autologous neutralization
Heterologous neutralization
Issue Date: 1997
Publisher: Rapid Science Publishers | Lippincott-Raven Publishers
Citation: BONGERTZ, Vera et al. Correlation between susceptibility of primary HIV-1 isolates to autologous and heterologous neutralizing antibodies. AIDS, v. 11, n. 8 p. 969-975, 1997.
Description: Hospital Evandro Chagas AIDS Clinical Research Group: S. Cavalcante; M.C.G. Galhardo; M.R.C. Guimarães; B. Grinsztejn and V.C. Rolla.
ISSN: 0269-9370
Copyright: restricted access
Appears in Collections:BA - IGM - Artigos de Periódicos
IOC - Artigos de Periódicos
INI - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Correlation_Valdilea_Veloso_etal_INI_1997.pdf31.47 MBAdobe PDF    Request a copy


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.