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2022-01-01
Sustainable Development Goals
03 Saúde e Bem-EstarCollections
- IOC - Artigos de Periódicos [12973]
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PHENOTYPIC AND GENOTYPIC CHARACTERIZATION OF DRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS STRAINS
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Abstract
Of 142 pulmonary tuberculosis patients, 76 were considered high risk for the development of resistance, and 24 were confirmed as
resistant strain carriers. Resistant isoniazid strains presented a high frequency of katG and ahpC mutations (90%) correlated with an MIC N4
μg/mL (94%). inhA mutations were not seen. rpoB mutations were identified in 78.6% of rifampicin-resistant strains, usually in codon 531
(72.7%), and 75% had an MIC N16 μg/mL. katG and rpoB mutations recognized 88.2% of multidrug-resistant strains and proved more
efficient than the katG and rpoB mutations alone. Seventy percent of resistant pyrazinamide strains had pncA mutations between genes 136
and 188, 62.5% of them with an MIC N900 μg/mL. Pyrazinamidase inactivity was not an efficient resistance marker because 60% of pncAmutated
strains maintained enzymatic activity despite displaying good correlation with high resistance levels. Resistant ethambutol strains
had embB mutations in codon 306, with MIC N16 μg/mL.
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