Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32090
Title: Trypanosoma cruzi alters adherens junctions in cardiomyocytes
Authors: Melo, Tatiana G. de
Meirelles, Maria de Nazareth S. L.
Pereira, Mirian Claudia S.
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ. Brasil.
Abstract: We analyzed the distribution and expression of cadherin and b-catenin during Trypanosoma cruziecardiomyocyte interaction. Confocal microscopy revealed cadherin associated with b-catenin at the cellecell contacts. After 24 h of infection, the spatial distribution and expression of both adherens junction (AJ) proteins remained unaltered. In contrast, loss of N-cadherinecatenin complex was visualized in highly infected cardiomyocytes. Immunoblotting assays corroborated the spatial disorder, showing a 46% reduction in both N-cadherin and b-catenin expression at later infection (72 h of infection). Our data demonstrate that T. cruzi infection disturbs AJs, which can result in loss of cardiac tension and may contribute to the cardiac dysfunctions present in T. cruzi infection.
Keywords: Trypanosoma cruzi
Cardiomyocytes
Adherens junctions
Cadherin
b-Catenin
keywords: Trypanosoma cruzi
DeCS: beta Catenina
Junções Aderentes
Caderinas
Miócitos Cardíacos
Issue Date: 2008
Publisher: Elsevier
Citation: MELO, Tatiana G. de; MEIRELLES, Maria de Nazareth S. L.; PEREIRA, Mirian Claudia S. Trypanosoma cruzi alters adherens junctions in cardiomyocytes. Microbes and Infection, v. 10, p. 1405-1410, 2008.
DOI: 10.1016/j.micinf.2008.07.044
ISSN: 1286-4579
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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