Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32217
Title: SOCS in situ expression in tuberculous lymphadenitis in an endemic area
Authors: Nicol, A. F.
Nuovo, G. J.
Coelho, J. M. C.
Rolla, V. C.
Horn, C.
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunopatologia. Rio de Janeiro, RJ, Brasil.
University Hospitals. Department of Pathology. Columbus, OH, USA.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micobactéria. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Imunologia. Rio de Janeiro, RJ, Brasil.
Abstract: Background: The immune response to Mycobacterium tuberculosis is complex and multifactorial, the cytokine system being a major factor in M. tuberculosis immunity. Aim: To analyze the immunohistochemical aspects of tuberculous lymph nodes in immunocompetent patients and search for associations between SOCS and cytokine expression in human tuberculous lymphadenitis. Methods: Thirteen lymph nodes were assayed by immunohistochemistry for SOCS-1 and 3, STAT-3, RANTES, MIP-1-α, ICAM-1, IFN-γ as well as CD45RO, CD20, CD34, CD68, trypsin and lysozyme. Additionally, the RT in situ PCR was performed for SOCS-1 and 3 mRNA detection. Results: Decreased MIP-1α expression together with reduced SOCS-3 (p= 0.042), lysozyme (p= 0.024) and CD45RO (p= 0.05) was observed in the TB lymph nodes compared to the control lymph nodes. In conclusion, the lymphadenitis due to M. tuberculosis was associated with a downregulation of memory T cells (CD45RO), activated lysozymes and SOCS-3 compared to controls, which may play a role in the long-term bacterial replication and altered immune modulation characteristic of the disease.
Keywords: Tuberculosis
Lymph Nodes
Human
SOCS
Immunophenotype
keywords: Tuberculose
Humanos
Linfonodos
Imunofenótipo
Proteínas Supressoras da Sinalização de Citocinas
DeCS: Proteínas Supressoras da Sinalização de Citocinas
Issue Date: 2008
Publisher: Elsevier
Citation: NICOL, A. F. et al. SOCS in situ expression in tuberculous lymphadenitis in an endemic area. Experimental and Molecular Pathology, v. 84, p. 240–244, 2008.
DOI: 10.1016/j.yexmp.2008.04.001
ISSN: 0014-4800
Copyright: restricted access
Appears in Collections:INI - Artigos de Periódicos
IOC - Artigos de Periódicos

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