Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/3240
Title: Role of costimulatory molecules in immune response of patients with cutaneous leishmaniasis
Authors: Favali, Cecilia Beatriz Fiuza
Costa, Dirceu Joaquim
Afonso, Lilian Maria
Conceição, Viviane
Rosato, Andréa Bomura
Oliveira, Fabiano
Costa, Jackson Mauricio Lopes
Barral, Aldina Maria Prado
Barral Netto, Manoel
Brodskyn, Claudia Ida
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Abstract: T cell-mediated immunity is critical in resistance against Leishmania parasites, and T cell activation requires signals provided by costimulatory molecules. Herein we evaluated the role of costimulatory molecules on cytokine production and T cell surface molecule expression by peripheral blood mononuclear cells (PBMC) from cutaneous leishmaniasis (CL) patients. PBMC from CL patients were stimulated with soluble Leishmania antigen (SLA, 10 microg/ml), in the presence or absence of soluble CTLA4-Ig to block CD28-B7 interaction or in the presence or absence of anti-human CD40L to block CD40-CD40L interaction. Supernatants were harvested to evaluate tumor necrosis factor alpha (TNF-alpha), interleukin 10 (IL-10), transforming growth factor beta (TGF-beta) and interferon gamma (IFN-gamma) production by ELISA. Cells were harvested after 48 h of culture, stained for specific activation markers and analyzed by flow cytometry. Results show that the blockade of CD28-B7 interaction by CTLA4-Ig downmodulated IFN-gamma, IL-10, and TNF-alpha secretion by PBMC from CL patients. No alteration was detected on either TGF-beta production or the expression of CTLA44 or CD25 on CD4+ and CD8+ T cells. When the CD40-CD40L interaction was blockade using anti-CD40L, we did not observe changes in cytokine production or in surface molecule expression. The blockade of the CD28-B7 interactions by CTLA4-Ig also did not alter cytokine production in volunteers immunized against tetanus toxoid (TT). Taken together, these data suggest that the interaction of CTLA4 and CD28-B7 is a TGF-beta-independent mechanism that specifically downmodulates the immune response in cutaneous leishmaniasis patients.
Keywords: Cutaneous leishmaniasis
Cytokines
Immunomodulation
DeCS: Antígenos
Células Cultivadas
Regulação para Baixo
Imunoconjugados
Interferon gama
Interleucina-10
Leishmaniose Cutânea
Leucócitos Mononucleares
Linfócitos T
Fator de Necrose Tumoral alfa
Feminino
Humanos
Issue Date: 2005
Citation: FAVALI, C. et al. Role of costimulatory molecules in immune response of patients with cutaneous leishmaniasis. Microbes and Infection, v.7, n.1, p.86-92, jan. 2005.
DOI: doi:10.1016/j.micinf.2004.09.015
ISSN: 1286-4579
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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