Detection of anti-Trypanosoma cruzi antibodies by chimeric antigens in chronic Chagas disease-individuals from endemic South American countries

Faculty of Technology and Sciences of Bahia. Salvador, BA, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Molecular Biology Institute of Paraná. Curitiba, PR, Brazil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Department of Pathology and Legal Medicine. Salvador, BA, Brazil / Yale University. School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, Connecticut, United States of America.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Institute for Research on Genetic Engineering and Molecular Biology “Dr Héctor Torres”. Laboratory of Molecular Biology of Chagas Disease. Buenos Aires, Argentina.
Institute for Research on Genetic Engineering and Molecular Biology “Dr Héctor Torres”. Laboratory of Molecular Biology of Chagas Disease. Buenos Aires, Argentina.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.

Abstract

Laboratory diagnosis of chronic Chagas disease is a troubling factor due to lack of reference tests. The WHO suggests the use of two distinct commercial serological tests in parallel. The performance of commercial immunoassays might fluctuate depending on the antigenic matrices and the local strains of T. cruzi in different geographical settings. The use of antigenic matrices based on chimeric proteins can solve these limitations. Here, we evaluated the diagnostic performance of two chimeric T. cruzi antigens (IBMP-8.1 and -8.4) to diagnose chronic Chagas disease in individuals from endemic South American countries.

Keywords in Portuguese

Trypanosoma cruzi
Doença de Chagas
Antígenos
Proteínas Quiméricas
Diagnóstico
Brasil

Keywords

Trypanosoma cruzi
Chagas disease
Antigens
Chimeric proteins
Diagnostics
Brazil

Publisher

Public Library of Science

Citation

DEL-REI, Rodrigo Pimenta et al. Detection of anti-Trypanosoma cruzi antibodies by chimeric antigens in chronic Chagas disease-individuals from endemic South American countries. Plos One, v. 14, n. 4, p. 1-12, 2019.

DOI

10.1371/journal.pone.0215623

ISSN

1932-6203

Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32985

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Copyright

Open access

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