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2022-01-01
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ALTERED EXPRESSION OF GALECTIN-3 INDUCES CORTICAL THYMOCYTE DEPLETION AND PREMATURE EXIT OF IMMATURE THYMOCYTES DURING TRYPANOSOMA CRUZI INFECTION
Monteiro, Elizangela Silva et al. | Date Issued: 2007
Author
Monteiro, Elizangela Silva
Lorenzato, Luciana Reis
Kenji Nihei, Oscar
Junqueira, Mara
Rabinovich, Gabriel Adrián
Hsu, Daniel Kaiyuan
Liu, Fu-Tong
Savino, Wilson
Chammas, Roger
Villa-Verde, Déa Maria Serra
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.
Universidade de São Paulo. Faculdade de Medicina. Laboratório de Oncologia Experimental. São Paulo, SP, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
Universidade de Buenos Aires. Faculdade de Medicina. Hospital de Clínicas José de San Martin. Divisão de Imunogenética. Buenos Ires, Argentina.
University of California. Department of Dermatology. Los Angeles, CA, USA.
University of California. Department of Dermatology. Los Angeles, CA, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.
Universidade de São Paulo. Faculdade de Medicina. Laboratório de Oncologia Experimental. São Paulo, SP, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.
Abstract
During acute infection with Trypanosoma cruzi, the causative agent of Chagas' disease, the thymus undergoes intense atrophy followed by a premature escape of CD4+CD8+ immature cortical thymocytes. Here we report a pivotal role for the endogenous lectin galectin-3 in accelerating death of thymocytes and migration of these cells away from the thymus after T. cruzi infection. We observed a pronounced increase in galectin-3 expression that paralleled the extensive depletion of CD4+CD8+ immature thymocytes after infection. In vitro, recombinant galectin-3 induced increased levels of death in cortical immature thymocytes. Consistent with the role of galectin-3 in promoting cell death, thymuses from gal-3-/- mice did not show cortical thymocyte depletion after parasite infection in vivo. In addition, galectin-3 accelerated laminin-driven CD4+CD8+ thymocyte migration in vitro and in vivo induced exportation of CD4+CD8+ cells from the thymus to the peripheral compartment. Our findings provide evidence of a novel role for galectin-3 in the regulation of thymus physiology and identify a potential mechanism based on protein-glycan interactions in thymic atrophy associated with acute T. cruzi infection.
Keywords in Portuguese
Trypanosoma cruzi
Infecção
Galacteina-3
Depleção timocortical cortical
Timócitos imaturos
Atrofia do Timo
 
Keywords
Trypanosoma cruzi
Infection
Galectin-3
Cortical Thymocyte Depletion
Thymic atrophy
Immature Thymocytes
 
Publisher
Elsevier
Citation
MONTEIRO, Elizangela Silva et al. Altered Expression of Galectin-3 Induces Cortical Thymocyte Depletion and Premature Exit of Immature Thymocytes during Trypanosoma cruzi Infection. The American Journal of Pathology, v. 170, n. 2, p. 546-556, Feb. 2007.
DOI
10.2353/ajpath.2007.060389
ISSN
0002-9440