Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/36201
Title: Immunodetection of toxins in historesin-embedded sections of Phoneutria nigriventer venom glands using laser confocal scanning microscopy
Authors: Silva, Luciana Maria
Pimenta, Rafael Nacif
Ortolani, Paula Ladeira
Moura, Layla Mosqueira
Cordeiro, Marta do Nascimento
Chavez-Olórtegui, Carlos
Pimenta, Paulo Filemon Paolucci
Fortes-Dias, Consuelo Latorre
Affilliation: Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brasil.
Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brasil.
Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brasil.
Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brasil.
Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brasil.
Abstract: In the last decades, main advances were achieved in the identification, structural and pharmacological characterization of Phoneutria nigriventer toxins. However, studies on the venom-producing apparatus are rare. Presently, we applied immunolabeling to historesin-embedded cross-sections of P. nigriventer venom glands. Toxins and toxin-secreting cells were successfully located in situ, using laser confocal scanning microscopy. The methodological strategy was successful and may be applied in future studies on venom glands and other secreting tissues, in general.
Keywords: Armed spider
Phoneutria nigriventer
Toxin immunodetection
Venom gland
Venom toxin secretion
Issue Date: 2019
Publisher: Elsevier Ltd
Citation: SILVA, Luciana Maria et al. Immunodetection of toxins in historesin-embedded sections of Phoneutria nigriventer venom glands using laser confocal scanning microscopy. Toxicon, v. 167, p. 168-171, 2019.
DOI: 10.1016/j.toxicon.2019.06.018
ISSN: 0041-0101
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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