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LEISHMANIA MAJOR: PARASITE INTERACTIONS SUGGESTING SEXUALITY
Leishmania major
Sexualidade
Morfologia
Imunocitoquímica
Microscopia eletrônica de varredura
Leishmania major
Sexuality
Immunocytochemistry
Scanning electron microscopy
Morphology
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Coleção de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil.
Abstract
In five experiments, Leishmania (Leishmania) major (MRHO/SU/59/P-strain) grew poorly when seeded in FYTS medium supplemented with 15% fetal calf serum, but presented several peculiar pairs of promastigotes diametrically opposed and attached at their posterior ends (5.8-13.5%). As seen in Giemsa-stained smears, a ring-like line and/or an enlargement, generally occurred at the parasite junction. A close proximity of nuclei, which sometimes were difficult to distinguish from each other, was also observed at this junction. Several of these pairs appeared to be composed of fused cells in which the nuclei could be apparently fused, as shown by fluorescence microscopy to detect ß-tubulin and DNA, and by scanning electron microscopy. Under other culture conditions these pairs were absent or occurred at very low rates (0.2-2.2%). Such pairs differ markedly from longitudinally dividing cells and resemble those described in two other Leishmania species, as well as in Herpetomonas megaseliae and Phytomonas davidi, suggesting steps of a putative sexual process.
Keywords in Portuguese
TrypanosomatidaeLeishmania major
Sexualidade
Morfologia
Imunocitoquímica
Microscopia eletrônica de varredura
Keywords
TrypanosomatidaeLeishmania major
Sexuality
Immunocytochemistry
Scanning electron microscopy
Morphology
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