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https://www.arca.fiocruz.br/handle/icict/3783
EXPERIMENTAL LEPTOSPIROSIS IN MARMOSET MONKEYS (CALLITHRIX JACCHUS): A NEW MODEL FOR STUDIES OF SEVERE PULMONARY LEPTOSPIROSIS
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Abstract
Experimental infection of marmoset monkeys (Callithrix jacchus) with Leptospira interrogans serovar
Copenhageni showed microscopic patterns of tissue reactions comparable to those seen in the severe forms of human
leptospirosis, including intra-alveolar hemorrhage. The most impressive microscopic changes were seen in the lung and
kidney of animals killed at days 6 and 12 after inoculation. There were extensive and irregular areas of hemorrhage
predominating around main bronchial branches or diffusely spread to the pulmonary parenchyma, as well as severe
tubulointerstitial nephritis. Antibody response detected by the microscopic agglutination test was quantitatively similar
to those seen in humans and paralleled severity of tissue lesions. The distribution of leptospires or antigenic debris in
infected tissues was observed by immunofluorescence and confocal laser scanning microscopy. Large numbers of typical
leptospires were seen in the lumen of proximal renal tubules. Positive reactions showing antigenic debris were closely
associated with sites of tissue damage.
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