Low-level laser therapy can reduce lipopolysaccharide-induced contractile force dysfunction and TNF-alpha levels in rat diaphragm muscle

Research Group of Animal Experimental, IP & D UNIVAP R. Shishima Hifumi. São José dos Campos, SP, Brasil.
Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Farmacologia. Laboratório de Farmacologia e Fototerapia da Inflamação. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Rio de Janeiro, RJ, Brasil.
Research Group of Animal Experimental, IP & D UNIVAP R. Shishima Hifumi. São José dos Campos, SP, Brasil.
Instituto do Coração. Departamento de Laser em Biomedicina. São Paulo, SP, Brasil.
Research Group of Animal Experimental, IP & D UNIVAP R. Shishima Hifumi. São José dos Campos, SP, Brasil.
Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Farmacologia. Laboratório de Farmacologia e Fototerapia da Inflamação. São Paulo, SP, Brasil.
University of Bergen. Haukeland University Hospital. Department of Biomedicine. Section of Physiology. Bergen, Norway.
University of Bergen. Institute of Public Health and Primary Health Care. Section of Physiotherapy Science. Section of `shysiotherapy Science. Bergen, Norway / Bergen University College, Physiotherapy Department. Bergen, Norway,

Abstract

Our objective was to investigate if low-level laser therapy (LLLT) could improve respiratory function and inhibit tumor necrosis factor (TNF-alpha) release into the diaphragm muscle of rats after an intravenous injection of lipopolysaccharide (LPS) (5 mg/kg). We randomly divided Wistar rats in a control group without LPS injection, and LPS groups receiving either (a) no therapy, (b) four sessions in 24 h with diode Ga-AsI-Al laser of 650 nm and a total dose of 5.2 J/cm2, or (c) an intravenous injection (1.25 mg/kg) of the TNF-alpha inhibitor chlorpromazine (CPZ). LPS injection reduced maximal force by electrical stimulation of diaphragm muscle from 24.15+/-0.87 N in controls, but the addition of LLLT partly inhibited this reduction (LPS only: 15.01+/-1.1 N vs LPS+LLLT: 18.84+/-0.73 N, P<0.05). In addition, this dose of LLLT and CPZ significantly (P<0.05 and P<0.01, respectively) reduced TNF-alpha concentrations in diaphragm muscle when compared to the untreated control group.

Keywords in Portuguese

Doença inflamatória crônica
Estimulação elétrica muscular
Clorpromazina
Laser de diodo Ga – AsI – Al

Keywords

Diode Ga–AsI–Al laser
Chronic inflammation disease
Chlorpromazine (CPZ)
Electrical muscle stimulation

Publisher

Springer Verlag (Germany)

Citation

AIMBIRE, F. et al. Low-level laser therapy can reduce lipopolysaccharide-induced contractile force dysfunction and TNF-α levels in rat diaphragm muscle. Lasers in Medical Science, v. 21, p. 238-244, 2006.

DOI

10.1007/s10103-006-0405-y

ISSN

0268-8921

Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/38717

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Copyright

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Embargo date

2025-01-01

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