Efficient in vitro expansion of JC virus-specific CD8+ T-cell responses by JCV peptide-stimulated dendritic cells from patients with progressive multifocal leukoencephalopathy

Harvard Medical School. Beth Israel Deaconess Medical Center. Division of Viral Pathogenesis. Boston, MA, USA.
Harvard Medical School. Beth Israel Deaconess Medical Center. Department of Neurology. Boston, MA, USA / Harvard Medical School. Beth Israel Deaconess Medical Center. Division of Viral Pathogenesis. Boston, MA, USA / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil.
Harvard Medical School. Beth Israel Deaconess Medical Center. Division of Viral Pathogenesis. Boston, MA, USA.
Harvard Medical School. Massachusetts General Hospital. Partners AIDS Research Center. Boston, MA, USA.
Harvard Medical School. Massachusetts General Hospital. Partners AIDS Research Center. Boston, MA, USA.
New York University. School of Medicine. New York, NY, USA.
New York University. School of Medicine. New York, NY, USA.
Harvard Medical School. Beth Israel Deaconess Medical Center. Department of Neurology. Boston, MA, USA / Harvard Medical School. Beth Israel Deaconess Medical Center. Division of Viral Pathogenesis. Boston, MA, USA.

Abstract

Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the brain caused by JC virus (JCV) for which there is no cure. PML patients who have JCV-specific CD8+ cytotoxic T lymphocytes (CTL) in their blood have a better clinical outcome. We compared JCV-specific CTL responses in vitro elicited either by JCV peptide-loaded dendritic cells (DC) or by direct peptide stimulation of lymphocytes from 20 HLA A*0201+ healthy controls, HIV+ and PML patients. JCV peptide-loaded DC elicited a stronger CTL expansion in 13/15 responders. DC can induce potent JCV-specific CTL response in vitro, and may constitute a promising approach for PML immunotherapy.

Keywords

JC virus
Progressive multifocal leukoencephalopathy
Dendritic cells

Publisher

Elsevier

Citation

MARZOCCHETTI, Angela et al. Efficient in vitro expansion of JC virus-specific CD8+ T-cell responses by JCV peptide-stimulated dendritic cells from patients with progressive multifocal leukoencephalopathy. Virology, v. 383, n. 2, p. 173-177, Jan. 2009.

DOI

10.1016/j.virol.2008.10.046

ISSN

0042-6822

Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/38791

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Open access

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