| Author | Prado, Roberta Oliveira | |
| Author | Caldas, Iramaya Rodrigues | |
| Author | Carvalho, Andrea Teixeira de | |
| Author | Andrade, Marcus Vinicius | |
| Author | Gazzinelli, Andréa | |
| Author | Oliveira, Rodrigo Correa de | |
| Author | Melo, Jose Renan da Cunha | |
| Access date | 2020-07-08T14:08:38Z | |
| Available date | 2020-07-08T14:08:38Z | |
| Document date | 2009 | |
| Citation | OLIVEIRA-PRADO, R. et al. CD4 and CD8 distribution profile in individuals infected by Schistosoma mansoni. Scandinavian Journal of Immunology, [Oslo], v. 69, p. 521–528, 2009. | pt_BR |
| ISSN | 0300-9475 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/42160 | |
| Language | por | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | restricted access | pt_BR |
| MeSH | Antigens, Helminth | pt_BR |
| MeSH | CD28 Antigens | pt_BR |
| MeSH | CD4-Positive T-Lymphocytes | pt_BR |
| MeSH | CD8-Positive T-Lymphocytes | pt_BR |
| MeSH | Flow Cytometry | pt_BR |
| MeSH | HLA-DR Antigens | pt_BR |
| MeSH | Mitogen-Activated Protein Kinase 1 | pt_BR |
| MeSH | Mitogen-Activated Protein Kinase 3 | pt_BR |
| MeSH | Phosphorylation | pt_BR |
| MeSH | Schistosoma mansoni | pt_BR |
| MeSH | Signal Transduction | pt_BR |
| MeSH | T-Lymphocyte Subsets | pt_BR |
| Title | CD4 and CD8 distribution profile in individuals infected by Schistosoma mansoni | pt_BR |
| Type | Article | |
| DOI | 10.1111/j.1365-3083.2009.02247.x | |
| Abstract | Rationale: Patients with chronic Schistosoma mansoni infection show lower antisoluble egg antigen (SEA) proliferation responses and higher responses to soluble worm antigen preparation (SWAP). Objective: To compare the activation status and proliferation response of peripheral blood mononuclear cells (PBMC) of infected (XTO) and egg-negative individuals (NI) living in the same endemic area. Methods: XTO (n = 51) and NI individuals from the same geographical area (n = 37) and healthy blood donors (n = 22) were evaluated before and after stimulation with SEA and SWAP. The expression of activation markers (CD4+ HLADR+, CD8high+HLA-DR+ and CD8+ CD28+) and proliferation assay was assessed by flow cytometry. Findings: PBMC from infected patients showed lower frequency of CD4+ but no change in CD8+ T cells when compared with the healthy donor group. The ratio CD4+ ⁄CD8+ was 1.3, 0.6 and 0.5 in healthy donors, infected and non-infected individuals, respectively. The HLA-DR+ expression on CD8+ was higher in PBMC from infected and noninfected individuals than from healthy donors, but similar in both total lymphocytes and CD4+ populations. No intergroup proliferation response differences were observed in CD4+ and CD8+ PBMC unstimulated and stimulated with SEA and SWAP. The SEA but not SWAP-stimulated cells showed a decrease in the expression of phosphorylated extracellular signal-regulated kinase (ERK1⁄ 2). Conclusions: XTO and NI individuals living in the same area presented a smaller per cent of CD4+ and a higher per cent of CD8+ cells. The activation by either CD8high+HLA-DR+ or CD8high+HLA-DR+ ⁄CD8+ was enhanced and decreased in XTO and NI by CD8+ CD28+ and CD8+ CD28+ ⁄CD8+ when compared with healthy donor. ERK phosphorylation was attenuated in XTO and NI individuals when stimulated with SEA but not SWAP. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Patologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Fiocruz Brasília. Brasília, DF, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Medicina Interna. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Escola de Enfermagem. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Cirurgia. Belo Horizonte, MG, Brasil. | pt_BR |
| Subject | HLA-DR antigens | pt_BR |
| Subject | CD28 antigens | pt_BR |
| Subject | Mitogen-Activated Protein Kinase | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| DeCS | Antígenos de Helmintos | pt_BR |
| DeCS | Antígenos CD28 | pt_BR |
| DeCS | Linfócitos T CD4-Positivos | pt_BR |
| DeCS | Linfócitos T CD8-Positivos | pt_BR |
| DeCS | Citometria de Fluxo | pt_BR |
| DeCS | Antígenos HLA-DR | pt_BR |
| DeCS | Proteína Quinase 1 Ativada por Mitógeno | pt_BR |
| DeCS | Proteína Quinase 3 Ativada por Mitógeno | pt_BR |
| DeCS | Schistosoma mansoni | pt_BR |
| Embargo date | 2025-12-31 | |
