Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/44559
Title: Isolation of a fragment homologous to the rp49 constitutive gene of Drosophila in the Neotropical malaria vector Anopheles aquasalis (Diptera: Culicidae)
Authors: Gentile, C.
Lima, J. B. P.
Peixoto, A. A.
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Entomologia. Rio de Janeiro, RJ, Brasil / Instituto de Biologia do Exército. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Abstract: The constitutive ribosomal gene rp49 is frequently used as an endogenous control in Drosophila gene expression experiments. Using the degenerate primer PCR technique we have cloned a fragment homologous to this gene in Anopheles aquasalis Curry, a Neotropical vector of malaria. In addition, based on this first sequence, a new primer was designed, which allowed the isolation of fragments of rp49 in two other species, Aedes aegypti (Linnaeus) and Culex quinquefasciatus Say, suggesting that it could be used to clone fragments of this gene in a number of other mosquito species. Primers were also designed to specifically amplify rp49 cDNA fragments in An. aquasalis and Ae. aegypti, showing that rp49 could be used as a good constitutive control in gene expression studies of these and other vectorially important mosquito species.
Keywords: Anopheles aquasalis
Aedes aegypti
Culex quinquefasciatus
Drosophila constitutive gene
rp49
rpL32
Mosquitoes
keywords: Anopheles aquasalis
Aedes aegypti
Culex quinquefasciatus
Drosophila constitutive gene
rp49
rpL32
Mosquitos
Issue Date: 2005
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
Citation: GENTILE, C.; LIMA, J. B. P.; PEIXOTO, A. A. Isolation of a fragment homologous to the rp49 constitutive gene of Drosophila in the Neotropical malaria vector Anopheles aquasalis (Diptera: Culicidae). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 6, p. 545-547, Oct. 2005.
DOI: 10.1590/S0074-02762005000600008
ISSN: 0074-0276
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos
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