| Author | Bezerra, Matheus Filgueira | |
| Author | Machado, Lais Ceschini | |
| Author | Carvalho, Viviane do Carmo Vasconcelos de | |
| Author | Docena, Cássia | |
| Author | Brandão-Filho, Sinval Pinto | |
| Author | Ayres, Constância Flávia Junqueira | |
| Author | Paiva, Marcelo Henrique Santos | |
| Author | Wallau, Gabriel Luz | |
| Access date | 2021-05-06T13:12:38Z | |
| Available date | 2021-05-06T13:12:38Z | |
| Document date | 2021 | |
| Citation | BEZERRA, Matheus Filgueira et al. A Sanger-based approach for scaling up screening of SARS-CoV-2 variants of interest and concern. medRxiv: The Preprint Server for Health Sciences, p. 1-12, 25 Mar. 2021. | |
| URI | https://www.arca.fiocruz.br/handle/icict/47064 | |
| Description | Este preprint relata novas pesquisas que não foram certificadas por revisão por pares e não devem ser usadas para orientar a prática clínica. | pt_BR |
| Description | A Rede Genômica Fiocruz é formada por especialistas de todas as unidades da Fundação no país e de institutos parceiros que se empenham diariamente em gerar dados mais robustos sobre o comportamento do SARS-Cov-2 e contribuir para um melhor preparo do país no enfrentamento da pandemia em termos de diagnóstico mais precisos e vacinas eficazes. Saiba mais sobre a Rede Genômica Fiocruz em: http://www.genomahcov.fiocruz.br/ | pt_BR |
| Sponsorship | CNPq | |
| Language | eng | en_US |
| Later version | https://www.arca.fiocruz.br/handle/icict/52140 | |
| Rights | restricted access | |
| Subject in Portuguese | Variantes preocupantes | pt_BR |
| Subject in Portuguese | Sequenciamento Sanger | pt_BR |
| Subject in Portuguese | Vigilância molecular | pt_BR |
| Subject in Portuguese | COVID-19 | pt_BR |
| Subject in Portuguese | SARS-CoV-2 | pt_BR |
| Subject in Portuguese | Rede Genômica Fiocruz | pt_BR |
| Subject in Portuguese | GENOMAHCOV | pt_BR |
| Title | A Sanger-based approach for scaling up screening of SARS-CoV-2 variants of interest and concern | en_US |
| Type | Preprint | |
| DOI | 10.1101/2021.03.20.21253956 | |
| Abstract | The global spread of new SARS-CoV-2 variants of concern underscore an urgent need of simple deployed molecular tools that can differentiate these lineages. Several tools and protocols have been shared since the beginning of the COVID-19 pandemic, but they need to be timely adapted to cope with SARS-CoV-2 evolution. Although whole-genome sequencing (WGS) of the virus genetic material have been widely used, it still presents practical difficulties such as high cost, shortage of available reagents in the global market, need of a specialized laboratorial infrastructure and well-trained staff. These limitations result in genomic surveillance blackouts across several countries. Here we propose a rapid and accessible protocol based on Sanger sequencing of a single PCR fragment that is able to identify and discriminate all SARS-CoV-2 variants of concern (VOCs) identified so far, according to each characteristic mutational profile at the Spike-RBD region (K417N/T, E484K, N501Y, A570D). Twelve COVID-19 samples from Brazilian patients were evaluated for both WGS and Sanger sequencing: three from P.2, two from P.1 and seven from B.1.1 lineage. All results from the Sanger sequencing method perfectly matched the mutational profile of VOCs and non-VOCs described by WGS. In summary, this approach allows a much broader network of laboratories to perform molecular surveillance of SARS-CoV-2 VOCs and report results within a shorter time frame, which is of utmost importance in the context of rapid public health decisions in a fast evolving worldwide pandemic. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia e Núcleo de Bioinformática. Recife, PE, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Núcleo de Plataforma Tecnológica. Recife, PE, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Núcleo de Plataforma Tecnológica. Recife, PE, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Recife, PE, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia e Núcleo de Bioinformática. Recife, PE, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia e Núcleo de Bioinformática. Recife, PE, Brasil / Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Núcleo de Ciências da Vida. Caruaru, PE, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia e Núcleo de Bioinformática. Recife, PE, Brasil. | |
| Subject | Variants of concern | en_US |
| Subject | Sanger sequencing | en_US |
| Subject | Molecular surveillance | en_US |
| Subject | COVID-19 | en_US |
| Subject | SARS-CoV-2 | en_US |
| Embargo date | 2030-03-25 | |
| Peer reviewed | Sim | |
| Publication status | Publicado | |
