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GEOGRAPHICAL ORIGIN OF CHRONIC CHAGAS DISEASE PATIENTS IN BRAZIL IMPACTS THE PERFORMANCE OF COMMERCIAL TESTS FOR ANTI-T. CRUZI IGG
Doença de Chagas em humanos
Testes de diagnóstico sorológico
Imunoensaios
Padrões de referência biológica internacional
Human Chagas disease
Serological diagnostic tests
Immunoassays
International Biological Reference Standards
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Abstract
BACKGROUND Chagas disease, caused by Trypanosoma cruzi, affects nearly six million people worldwide. Various serological
tests have been developed for its diagnosis.
OBJECTIVE Examine the performance of a set of commercial immunological assays in relation to the geographical origin of the
patient sample comparing four states of Brazil: Amazonas (AM), Mato Grosso do Sul (MS), Minas Gerais (MG) and Piauí (PI).
METHODS Seven immunoassays were employed to detect anti-T. cruzi IgG antibodies in 379 patient samples that had been
previously diagnosed using the two-step protocol required by the Brazilian Ministry of Health.
FINDINGS A significant variation in the percent reactive was calculated for the samples from AM and MS, while the PI and MG
showed a significant variation in the percent non-reactive. The average reactivity index was significantly higher for samples from
the states of PI and MG states than AM and MS.
MAIN CONCLUSIONS All tests presented a satisfactory performance overall. Yet, variations were observed that were associated
to the region of origin of the samples. Our analyses suggest that future evaluations of immunoassays should include a sampling
of sera from regions where the test will be applied in addition to the available International Biological Reference Standards.
Keywords in Portuguese
Trypanosoma cruziDoença de Chagas em humanos
Testes de diagnóstico sorológico
Imunoensaios
Padrões de referência biológica internacional
Keywords
Trypanosoma cruziHuman Chagas disease
Serological diagnostic tests
Immunoassays
International Biological Reference Standards
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