Author | Fernandes, Priscilla Augusta de Sousa | |
Author | Silva, Josefa Carolaine Pereira da | |
Author | Sales, Débora Lima | |
Author | Ribeiro, Paulo Riceli Vasconcelos | |
Author | Brito, Edy Sousa de | |
Author | Kerntopf, Marta Regina | |
Author | Delmondes, Gyllyandeson de Araújo | |
Author | Pinheiro, Jacqueline Cosmo Andrade | |
Author | Salazar, Gerson Javier Torres | |
Author | Batista, Francisco Lucas Alves | |
Author | Magalhães, Francisco Ernani Alves | |
Author | Gomez, Maria Celeste Vega | |
Author | Rolón, Míriam | |
Author | Coronel, Cathia | |
Author | Ribeiro Filho, Jaime | |
Author | Bezerra, José Weverton Almeida | |
Author | Siyadatpanah, Abolghasem | |
Author | Nissapatorn, Veeranoot | |
Author | Pereira, Maria de Lourdes | |
Author | Coutinho, Henrique Douglas Melo | |
Author | Braga, Maria Flaviana Bezerra Morais | |
Access date | 2021-09-21T17:47:25Z | |
Available date | 2021-09-21T17:47:25Z | |
Document date | 2021 | |
Citation | FERNANDES, Priscilla Augusta de Sousa et al. Chemical Constituents and Biological Activities of Croton heliotropiifolius Kunth. Antibiotics, v. 10, 2021. | pt_BR |
ISSN | 2079-6382 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/49126 | |
Description | 1 Department of Biological Chemistry, Universidade Regional do Cariri—URCA, Av. Cel Antônio Luis, 1161,
Pimenta, Crato 63105-010, Brazil; priscilla.fernandes@urca.br (P.A.d.S.F.);
debora.lima.sales@gmail.com (D.L.S.); martareginakerntopfm@outlook.com (M.R.K.);
gyllyandesondelmondes@gmail.com (G.d.A.D.); timotygertor@yahoo.com (G.J.T.S.);
lucas-a-b-181@hotmail.com (F.L.A.B.)
2 Department of Biological Sciences, Universidade Regional do Cariri—URCA, Av. Cel Antônio Luis, 1161,
Pimenta, Crato 63105-010, Brazil; carol.bio1881@outlook.com (J.C.P.d.S.);
flavianamoraib@yahoo.com.br (M.F.B.M.-B.)
3 Embrapa Agroindústria Tropical, Tropical R. Pernambuco, 2270–Pici, Fortaleza 60511-110, Brazil;
paulo.riceli@embrapa.br (P.R.V.R.); edy.brito@embrapa.br (E.S.d.B.)
4 Laboratório de Bioensaios, Universidade Federal do Cariri—UFCA, R. Olegário Emídio de Araújo, s/n,
Centro, Brejo Santo 63260-000, Brazil; andradejacquelinec@gmail.com
5 Inhamuns Region Education, Science and Technology Center, Universidade Estadual do Ceará—UECE,
BR-020—Bezerra de Sousa, Tauá 63660-000, Brazil; fernanimagalhaes@yahoo.com.br
6 Center for the Development of Scientific Research, Asuncion 1255, Paraguay;
mcvegagomez@gmail.com (M.C.V.G.); rolonmiriam@gmail.com (M.R.)
7 Laboratório de Investigação em Genética e Hematologia Translacional, Instituto Gonçalo Moniz, FIOCRUZ,
Salvador 40296-710, Brazil; cathiacoronel@gmail.com (C.C.); jaimeribeirofilho@gmail.com (J.R.-F.)
8 Department of Botany, Universidade Federal de Pernambuco—UFPE, Av. Prof. Moraes Rego, 1235,
Recife 50670-901, Brazil; weverton.almeida@urca.br
9 Ferdows School of Paramedical and Health, Birjand University of Medical Sciences, Birjand 9717853577, Iran;
asiyadatpanah@yahoo.com
10 School of Allied Health Sciences, World Union for Herbal Drug Discovery (WUHeDD) and Research
Excellence Center for Innovation and Health Products (RECIHP), Walailak University,
Nakhon Si Thammarat 80160, Thailand; veeranoot.ni@wu.ac.th
11 CICECO-Aveiro Institute of Materials and Department of Medical Sciences, University of Aveiro,
3810-193 Aveiro, Portugal | pt_BR |
Sponsorship | Fundação Cearense de Apoio ao Desenvolvimento Científico
e Tecnológico—FUNCAP through a Master’s scholarship. | pt_BR |
Language | eng | pt_BR |
Publisher | MDPI | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Croton | pt_BR |
Subject in Portuguese | Compostos fenólicos | pt_BR |
Subject in Portuguese | Antioxidantes | pt_BR |
Subject in Portuguese | Cromatografia | pt_BR |
Subject in Portuguese | Antiparasitários | pt_BR |
Title | Chemical Constituents and Biological Activities of Croton heliotropiifolius Kunth | pt_BR |
Type | Article | pt_BR |
DOI | 10.3390/antibiotics10091074 | |
Abstract | Croton heliotropiifolius Kunth (Euphorbiaceae), whose occurrence has already been registered
in the most varied Brazilian biomes, is commonly found in the Chapada do Araripe, Ceará. The
species is traditionally used to treat fungal, parasitic, and degenerative diseases. This study investigated
the chemical composition and pharmacological potential (antioxidant, antifungal, antiparasitic,
and cytotoxic) of an aqueous extract obtained from the roots of C. heliotropiifolius. Following a qualitative
phytochemical screening, the chemical constituents were identified by ultra-efficiency liquid
chromatography coupled witha quadrupole/time-of-flight system (UPLC-QTOF). The antioxidant
potential was verified by thin-layer chromatography (TLC). The direct and combined antifungal
activity of the extract against opportunistic Candida strains was investigated using the microdilution
method. The minimal fungicidal concentration (MFC) was determined by subculture, while the
modulation of the morphological transition (fungal virulence) was evaluated by light microscopy.
The in vitro antiparasitic activity was analyzed using epimastigotes of Trypanosoma cruzi and promastigotes
of Leishmania braziliensis and Leishmania infantum, while cytotoxicity was determined in
cultures of mouse fibroblasts. The phytochemical analysis identified the presence of acids, terpenes, flavonoids, lignans, and alkaloids. Among these constituents, the presence of polar and non-polar
phenolic compounds with known antioxidant action was highlighted. While the extract showed
clinically ineffective antifungal effects, it could enhance the effectiveness of fluconazole, in addition
to inhibiting the morphological transition associated with increased virulence in Candida strains.
Although the extract showed low cytotoxicity against fibroblasts, it also had weak antiparasitic
effects. In conclusion, Croton heliotropiifolius is a source of natural products with antifungal and
antioxidant potential. | pt_BR |
Affilliation | “Múltipla – ver em notas” | pt_BR |
Subject | Croton heliotropiifolius | pt_BR |
Subject | Phenolic compounds | pt_BR |
Subject | Antioxidant | pt_BR |
Subject | Antifungal | pt_BR |
Subject | Cytotoxicity | pt_BR |