Please use this identifier to cite or link to this item:
https://www.arca.fiocruz.br/handle/icict/49126
CHEMICAL CONSTITUENTS AND BIOLOGICAL ACTIVITIES OF CROTON HELIOTROPIIFOLIUS KUNTH
Author
Fernandes, Priscilla Augusta de Sousa
Silva, Josefa Carolaine Pereira da
Sales, Débora Lima
Ribeiro, Paulo Riceli Vasconcelos
Brito, Edy Sousa de
Kerntopf, Marta Regina
Delmondes, Gyllyandeson de Araújo
Pinheiro, Jacqueline Cosmo Andrade
Salazar, Gerson Javier Torres
Batista, Francisco Lucas Alves
Magalhães, Francisco Ernani Alves
Gomez, Maria Celeste Vega
Rolón, Míriam
Coronel, Cathia
Ribeiro Filho, Jaime
Bezerra, José Weverton Almeida
Siyadatpanah, Abolghasem
Nissapatorn, Veeranoot
Pereira, Maria de Lourdes
Coutinho, Henrique Douglas Melo
Braga, Maria Flaviana Bezerra Morais
Silva, Josefa Carolaine Pereira da
Sales, Débora Lima
Ribeiro, Paulo Riceli Vasconcelos
Brito, Edy Sousa de
Kerntopf, Marta Regina
Delmondes, Gyllyandeson de Araújo
Pinheiro, Jacqueline Cosmo Andrade
Salazar, Gerson Javier Torres
Batista, Francisco Lucas Alves
Magalhães, Francisco Ernani Alves
Gomez, Maria Celeste Vega
Rolón, Míriam
Coronel, Cathia
Ribeiro Filho, Jaime
Bezerra, José Weverton Almeida
Siyadatpanah, Abolghasem
Nissapatorn, Veeranoot
Pereira, Maria de Lourdes
Coutinho, Henrique Douglas Melo
Braga, Maria Flaviana Bezerra Morais
Affilliation
“Múltipla – ver em notas”
Abstract
Croton heliotropiifolius Kunth (Euphorbiaceae), whose occurrence has already been registered
in the most varied Brazilian biomes, is commonly found in the Chapada do Araripe, Ceará. The
species is traditionally used to treat fungal, parasitic, and degenerative diseases. This study investigated
the chemical composition and pharmacological potential (antioxidant, antifungal, antiparasitic,
and cytotoxic) of an aqueous extract obtained from the roots of C. heliotropiifolius. Following a qualitative
phytochemical screening, the chemical constituents were identified by ultra-efficiency liquid
chromatography coupled witha quadrupole/time-of-flight system (UPLC-QTOF). The antioxidant
potential was verified by thin-layer chromatography (TLC). The direct and combined antifungal
activity of the extract against opportunistic Candida strains was investigated using the microdilution
method. The minimal fungicidal concentration (MFC) was determined by subculture, while the
modulation of the morphological transition (fungal virulence) was evaluated by light microscopy.
The in vitro antiparasitic activity was analyzed using epimastigotes of Trypanosoma cruzi and promastigotes
of Leishmania braziliensis and Leishmania infantum, while cytotoxicity was determined in
cultures of mouse fibroblasts. The phytochemical analysis identified the presence of acids, terpenes, flavonoids, lignans, and alkaloids. Among these constituents, the presence of polar and non-polar
phenolic compounds with known antioxidant action was highlighted. While the extract showed
clinically ineffective antifungal effects, it could enhance the effectiveness of fluconazole, in addition
to inhibiting the morphological transition associated with increased virulence in Candida strains.
Although the extract showed low cytotoxicity against fibroblasts, it also had weak antiparasitic
effects. In conclusion, Croton heliotropiifolius is a source of natural products with antifungal and
antioxidant potential.
Share