Author | Farias, Beatriz Oliveira | |
Author | Bianco, Kayo | |
Author | Nascimento, Ana Paula Alves | |
Author | Gonçalves de Brito, Andressa Silva | |
Author | Moreira, Thais Costa | |
Author | Clementino, Maysa Mandetta | |
Access date | 2022-03-24T12:04:40Z | |
Available date | 2022-03-24T12:04:40Z | |
Document date | 2022 | |
Citation | FARIAS, Beatriz Oliveira et al. Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants. Microbial Drug Resistance, v. 0, n. 0, p. 1-9, 2022. | pt_BR |
ISSN | 1931-8448 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/51850 | |
Description | A Rede Genômica Fiocruz é formada por especialistas de todas as unidades da Fundação no país e de institutos parceiros que se empenham diariamente em gerar dados mais robustos sobre o comportamento do SARS-Cov-2 e contribuir para um melhor preparo do país no enfrentamento da pandemia em termos de diagnóstico mais precisos e vacinas eficazes. Saiba mais sobre a Rede Genômica Fiocruz em: http://www.genomahcov.fiocruz.br/ | |
Language | eng | pt_BR |
Rights | open access | pt_BR |
Title | Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants | pt_BR |
Type | Article | pt_BR |
DOI | 10.1089/mdr.2021.0254 | |
Abstract | The emergence of vancomycin-resistant Enterococcus faecium (Efm) harboring vanA gene and multidrug-resistant determinants is a relevant public health concern. It is an opportunistic pathogen responsible for nosocomial infections widely distributed in the environment, including wastewater treatment plants (WWTPs). Our study addresses a genomic investigation of vanA-carrying Efm from WWTPs in Brazil. Samples from five WWTPs supplied with sewage from different sources were evaluated. Here we present whole-genome sequencing of eight vanA-Efm isolates performed on Illumina MiSeq platform. All these isolates presented multidrug-resistant profile, and five strains were from treated wastewater. Multiple antimicrobial resistance genes (ARGs) were found, such as aph(3′)-IIIa, ant(6′)-Ia, erm(B), and msrC, some of them being allocated in plasmids. The virulence profile was predominantly constituted by efaAfm and acm genes and all isolates, except for one, were predicted as human pathogens. Multilocus sequence typing analysis revealed a new allele and five different STs, three previously described (ST32, ST168, and ST253) and two novel ones (ST1893 and ST1894). Six strains belonged to CC17, often associated with hospital outbreaks. As far as our knowledge, no genomic studies of vanA-Efm recovered from WWTPs revealed isolates belonging to CC17 in Brazil. Therefore, our findings point to the environmental spread of Efm carrying multiple ARGs. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Vancomycin-resistant enterococci (VRE) | pt_BR |
Subject | Antibiotic resistance | pt_BR |
Subject | Whole-genome sequencing | pt_BR |
Subject | Wastewater | pt_BR |
DeCS | Enterococos Resistentes à Vancomicina | pt_BR |
DeCS | Resistência Microbiana a Medicamentos | pt_BR |
DeCS | Sequenciamento Completo do Genoma | pt_BR |
DeCS | Águas Residuárias | pt_BR |