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AEDES AEGYPTI AAG-2 CELL PROTEOME MODULATION IN RESPONSE TO CHIKUNGUNYA VIRUS INFECTION
Cultura de células de mosquito
Quantificação sem rótulo
Espectrometria de massa
Síntese proteíca
Apoptose
RNA helicases
Mitocôndria
Mosquito cell culture
Label-free quantification
Mass spectrometry
Protein synthesis
Apoptosis
RNA helicases
Mitochondrion
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Abstract
Chikungunya virus (CHIKV) is a single-stranded positive RNA virus that belongs to the
genus Alphavirus and is transmitted to humans by infected Aedes aegypti and Aedes
albopictus bites. In humans, CHIKV usually causes painful symptoms during acute and
chronic stages of infection. Conversely, virus–vector interaction does not disturb the
mosquito’s fitness, allowing a persistent infection. Herein, we studied CHIKV infection of
Ae. aegypti Aag-2 cells (multiplicity of infection (MOI) of 0.1) for 48 h through label-free
quantitative proteomic analysis and transmission electron microscopy (TEM). TEM images
showed a high load of intracellular viral cargo at 48 h postinfection (hpi), as well as an
unusual elongated mitochondria morphology that might indicate a mitochondrial
imbalance. Proteome analysis revealed 196 regulated protein groups upon infection,
which are related to protein synthesis, energy metabolism, signaling pathways, and
apoptosis. These Aag-2 proteins regulated during CHIKV infection might have roles in
antiviral and/or proviral mechanisms and the balance between viral propagation and the
survival of host cells, possibly leading to the persistent infection.
Keywords in Portuguese
Chikungunya virusCultura de células de mosquito
Quantificação sem rótulo
Espectrometria de massa
Síntese proteíca
Apoptose
RNA helicases
Mitocôndria
Keywords
CHIKVMosquito cell culture
Label-free quantification
Mass spectrometry
Protein synthesis
Apoptosis
RNA helicases
Mitochondrion
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