Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/5646
Title: HIV-1 subtyping in Salvador, Bahia, Brazil: a city with African sociodemographic characteristics
Authors: Fernandez, José Carlos Couto
Morgado, Mariza Gonçalves
Bongertz, Vera
Tanuri, Amilcar
Andrade, Tarcisio Mattos de
Alves, Carlos Roberto Brites
Castro Filho, Bernardo Galvão
Affilliation: Fundação Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil
Universidade Federal do Rio de Janeiro. Departamento de Genética. Rio de Janeiro, RJ, Brasil
Universidade Federal da Bahia. Centro de Tratamento do Abuso de Drogas. Salvador, BA, Brasil
Universidade Federal da Bahia. Laboratorio de Retrovirologia Prof. Edgard Santos Hospital. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Abstract: To investigate the prevalence of the HIV-1 subtypes in different populations from Salvador, Bahia, Brazil, blood samples from 72 HIV-1-seropositive injecting drug users (IDUs) and 62 individuals infected sexually were analyzed using the heteroduplex mobility assay (HMA). In the IDU group, 89.5% were classified as subtype B, 3% as subtype F, and 7.5% showed a B/F HMA profile. In the sexual transmission (ST) group, 95% were identified as B subtype, 3.4% showed a B/F profile, and 1.6% a B/C/E HMA profile. All Brazilian samples that showed multiple reactivities in the HMA analysis clustered on sequencing with B North American/ European HIV-1 isolates in the phylogenetic analysis, whereas the F subtypes clustered with F Brazilian HIV-I isolates. Serologic reactivities of IDU's sera were examined using a panel of synthetic V3 loop peptides representative of the different HIV-1 subtypes. No difference in serologic reactivity between F and B subtype plasma could be observed. Predominance of HIV-I subtype B was identified in both study groups, whereas subtype F was detected only among IDUs in a frequency lower than described for other Brazilian regions.
Keywords: HIV-1
Subtypes
Diversity
Brazil
DeCS: Variação Antigênica
Infecções por HIV/virologia
HIV-1/classificação
Adolescente
Adulto
África/etnologia
Grupo com Ancestrais do Continente Africano
Brasail
Ensaio de Imunoadsorção Enzimática
Feminino
Infecções por HIV/imunologia
Infecções por HIV/transmissão
HIV-1/imunologia
Análise Heteroduplex
Humanos
Masculino
Meia-Idade
Filogenia
Reação em Cadeia da Polimerase
Comportamento Sexual
Fatores Socioeconômicos
Abuso de Substâncias por Via Intravenosa/complicações
Issue Date: 1999
Citation: COUTO-FERNANDEZ, J.C. et al. HIV-1 subtyping in Salvador, Bahia, Brazil: a city with African sociodemographic characteristics. Journal of Acquired Immune Deficience Syndromes v. 22, n. 3, p. 288-93, nov. 1999.
ISSN: 1525-4135
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Couto-Fernandes HIV_1_Subtyping_in_Salvador,_Bahia,_Brazil__A_City.11[1].pdf4.17 MBAdobe PDFThumbnail
View/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.