Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/5708
Title: Molecular and biological diversity of HIV-1 in Brazil.
Authors: Fernandez, José Carlos Couto
Morgado, Mariza Gonçalves
Santos, Jairo Ivo dos
Castro Filho, Bernardo Galvão
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Abstract: To determine the genomic polymorphism and biological properties present in HIV-1 Brazilian isolates, we analyzed five viral isolates obtained from patients residing in Rio de Janeiro (P1 and P5), São Paulo (P3) and Bahia (P2 and P4) states. For each viral isolate in vitro characteristics such as replication rate, syncytium-inducing capacity and cell death were observed in lymphoblastoid (H9, CEM and peripheral blood mononuclear cells) as well as monocytoid (U937) cells. In addition, the evaluation of the restriction fragment length polymorphism of these isolates was also performed using a panel of endonucleases such as Hind III, Bgl II, Sac I, Pst I, Kpn I and Eco RI. One of the isolates (P1), showed the highest phenotypic and genotypic divergence, when compared to others. The results found suggest a HIV heterogeneity in Brazil similar to that already described in other regions of the world.
Keywords: Brazilian HIV
Virulence
Restriction analysis
Viral diversity
DeCS: DNA Viral/análise
Variação Genética
HIV-1/genética
Síndrome de Imunodeficiência
Adquirida/microbiologia
Brasil
HIV-1/patogenicidade
Humanos
Masculino
Polimorfismo Genético
Polimorfismo de Fragmento de Restrição
Mapeamento por Restrição
Virulência
Issue Date: 1992
Citation: COUTO-FERNANDEZ, J.C. et al. Molecular and biological diversity of HIV-1 in Brazil. Memórias do Instituto Oswaldo Cruz, v. 87, n. 2, p. 249-55, apr.-jun. 1992.
ISSN: 0074-0276
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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