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CIRRHOTIC LIVER SUSTAINS IN SITU REGENERATION OF ACELLULAR LIVER SCAFFOLDS AFTER TRANSPLANTATION INTO G-CSF-TREATED ANIMALS
Fígado descelularizado
Reação ductular
G-CSF
Recelularização in vivo
Cirrose hepática
Regeneração hepática
Engenharia de tecidos
Decellularized liver
Ductular reaction
G-CSF
In vivo recellularization
Liver cirrhosis
Liver regeneration
Tissue engineering
Author
Affilliation
Abstract
Acellular liver scaffolds (ALS) produced by decellularization have been successfully explored for distinct regenerative purposes. To date, it is unknown whether transplanted ALSs are affected by cirrhotic livers, either becoming cirrhotic themselves or instead remaining as a robust template for healthy cell growth after transplantation into cirrhotic rats. Moreover, little is known about the clinical course of recipient cirrhotic livers after ALS transplantation. To address these questions, we transplanted ALSs into cirrhotic rats previously treated with the granulocyte colonystimulating factor. Here, we report successful cellular engraftment within the transplanted ALSs at 7, 15, and 30 days after transplantation. Recellularization was orchestrated by liver tissue cell activation, resident hepatocytes and bile duct proliferation, and an immune response mediated by the granulocyte components. Furthermore, we showed that transplanted ALSs ensured a pro-regenerative and anti-inflammatory microenvironment, attracted vessels from the host cirrhotic tissue, and promoted progenitor cell recruitment. ALS transplantation induced cirrhotic liver regeneration and extracellular matrix remodeling. Moreover, the transplanted ALS sustained blood circulation and attenuated alterations in the ultrasonographic and biochemical parameters in cirrhotic rats. Taken together, our results confirm that transplanted ALSs are not affected by cirrhotic livers and remain a robust template for healthy cell growth and stimulated cirrhotic liver regeneration.
Keywords in Portuguese
Matriz extracelular descelularizadaFígado descelularizado
Reação ductular
G-CSF
Recelularização in vivo
Cirrose hepática
Regeneração hepática
Engenharia de tecidos
Keywords
Decellularized extracellular matrixDecellularized liver
Ductular reaction
G-CSF
In vivo recellularization
Liver cirrhosis
Liver regeneration
Tissue engineering
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