Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/59380

Type
Preprint
Copyright
Open access
Collections
Share

Statistics
Metadata
Show full item record

MEDIA MATTERS! ALTERATIONS IN THE LOADING AND RELEASE OF HISTOPLASMA CAPSULATUM EXTRACELLULAR VESICLES IN RESPONSE TO DIFFERENT NUTRITIONAL MILIEUS
Cleare, Levi G. et al. | Date Issued: 2020
Author
Cleare, Levi G.
Zamith, Daniel
Heyman, Heino M.
Couvillion, Sneha P.
Nimrichter, Leonardo
Rodrigues, Marcio L.
Nakayasu, Ernesto S.
Nosanchuk, Joshua D.
Affilliation
Department of Medicine (Division of Infectious Diseases) and Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, USA.
Department of Medicine (Division of Infectious Diseases) and Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, USA.
Department of Medicine (Division of Infectious Diseases) and Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, USA / Biological Sciences Division, Pacific Northwest National Laboratory. Richland. USA / Bruker Daltonics Inc. Billerica, USA.
Biological Sciences Division. Pacific Northwest National Laboratory. Richland, USA.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, Brazil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, Brazil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil.
Biological Sciences Division. Pacific Northwest National Laboratory. Richland, USA.
Department of Medicine (Division of Infectious Diseases) and Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, USA.
Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brazil.
Abstract
Histoplasma capsulatum is a dimorphic fungus that most frequently causes pneumonia, but can also disseminate and proliferate in diverse tissues. Histoplasma capsulatum has a complex secretion system that mediates the release of macromolecule-degrading enzymes and virulence factors. The formation and release of extracellular vesicles (EVs) are an important mechanism for non-conventional secretion in both ascomycetes and basidiomycetes. Histoplasma capsulatum EVs contain diverse proteins associated with virulence and are immunologically active. Despite the growing knowledge of EVs from H. capsulatum and other pathogenic fungi, the extent that changes in the environment impact the sorting of organic molecules in EVs has not been investigated. In this study, we cultivated H. capsulatum with distinct culture media to investigate the potential plasticity in EV loading in response to differences in nutrition. Our findings reveal that nutrition plays an important role in EV loading and formation, which may translate into differences in biological activities of these fungi in various fluids and tissues.
Keywords
BHI
Culture media
Dimorphic fungus
Extracellular vesicles
Ham's F12
Histoplasma capsulatum
Histoplasmosis
Lipidomics
Metabolomics
Proteomics
RPMI
USA
Histoplasma-capsulatum
Lipids
 
Publisher
Wiley-Blackwell
Later version
https://www.arca.fiocruz.br/handle/icict/59387
Citation
CLEARE, Levi G. et al. Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus. Cellular Microbiology, v. 22, n. 9, p. 1-32, 2020.
DOI
10.1111/CMI.13217
ISSN
1462-5814