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2034
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A MODULE FOR ANALYZING INTERACTOMES VIA APEX-MS INTEGRATED INTO PATTERNLAB FOR PROTEOMICS
Peroxidase
Interactoma
Interação proteína-proteína
Affilliation
Institut Pasteur de Montevideo. Instituto de Investigaciones Biológicas Clemente Estable. Analytical Biochemistry and Proteomics Unit. Montevideo, Uruguay / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica Estrutural e Computacional. Curitiba, PR, Brasil.
Institut Pasteur de Montevideo. Instituto de Investigaciones Biológicas Clemente Estable. Analytical Biochemistry and Proteomics Unit. Montevideo, Uruguay / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica Estrutural e Computacional. Curitiba, PR, Brasil.
Institut Pasteur de Montevideo. Instituto de Investigaciones Biológicas Clemente Estable. Analytical Biochemistry and Proteomics Unit. Montevideo, Uruguay / Universidad de la Republica. Facultad de Química. Montevideo, Uruguay.
Institut Pasteur de Montevideo. Instituto de Investigaciones Biológicas Clemente Estable. Analytical Biochemistry and Proteomics Unit. Montevideo, Uruguay.
Institut Pasteur de Montevideo. Instituto de Investigaciones Biológicas Clemente Estable. Analytical Biochemistry and Proteomics Unit. Montevideo, Uruguay / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica Estrutural e Computacional. Curitiba, PR, Brasil.
Institut Pasteur de Montevideo. Instituto de Investigaciones Biológicas Clemente Estable. Analytical Biochemistry and Proteomics Unit. Montevideo, Uruguay / Universidad de la Republica. Facultad de Química. Montevideo, Uruguay.
Institut Pasteur de Montevideo. Instituto de Investigaciones Biológicas Clemente Estable. Analytical Biochemistry and Proteomics Unit. Montevideo, Uruguay.
Abstract
Proximity labeling techniques, such as APEX-MS, provide valuable insights into proximal interactome mapping; however, the verification of biotinylated peptides is not straightforward. With this as motivation, we present a new module integrated into PatternLab for proteomics to enable APEX-MS data interpretation by targeting diagnostic fragment ions associated with APEX modifications. We reanalyzed a previously published APEX-MS data set and report a significant number of biotinylated peptides and, consequently, a confident set of proximal proteins. As the module is part of the widely adopted PatternLab for proteomics software suite, it offers users a comprehensive, easy, and integrated solution for data analysis. Given the broad utility of the APEX-MS technique in various biological contexts, we anticipate that our module will be a valuable asset to researchers, facilitating and enhancing interactome studies. PatternLab’s APEX, including a usage protocol, is available at http://patternlabforproteomics.org/apex.
Keywords in Portuguese
PatternLab para proteômicaPeroxidase
Interactoma
Interação proteína-proteína
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