Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/6805
Title: Phenotypic study of peripheral blood lymphocytes and humoral immune response in Helicobacter pylori infection according to age
Authors: Soares, Taciana de Figueiredo
Rocha, Gifone Aguiar
Rocha, Andreia Maria Camargos
Oliveira, Celso Afonso de
Queiroz, Dulciene Maria de Magalhães
Oliveira, Rodrigo Correa de
Martins Filho, Olindo Assis
Carvalho, Anfrisina Sales Teles de
Bittencourt, Paulo Fernando Souto
Faria, Ana Maria Caetano de
Affilliation: Universidade Federal de Minas Gerais. Laboratory of Research in Bacteriology. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Laboratory of Research in Bacteriology. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Laboratory of Research in Bacteriology. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Laboratory of Research in Bacteriology. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Laboratory of Research in Bacteriology. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Research Center Rene Rachou. Laboratory of Cellular and Molecular Immunology. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Research Center Rene Rachou. Laboratory of Chagas’ Diseases. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Department of Pediatrics. Belo Horizonte, MG, Brazil.
Hospital Felicio Rocho. Service of Endoscopy. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Department of Biochemistry and Immunology. Belo Horizonte, MG, Brazil.
Abstract: There are differences between children and adults in certain aspects of the Helicobacter pylori (HP) infection, among them the lower titre of IgG antibodies anti-HP in the former group. Thus, we investigated by means of flow cytometry CD4+/CD3+ (CD4+T), CD8+/CD3+ (CD8+T) and CD19+/CD3- (B) cells, activation/co-stimulatory markers (CD4+/HLA-DR+, CD4+/CD28+, CD8+/HLA-DR+ and CD8+/CD28+) and by means of ELISA IgG anti-HP antibodies in the peripheral blood from HP-positive and -negative children and adults. An increased CD4+/CD28+ and CD8+/CD28+ percentage and number of CD4+/CD3+ cells were seen in infected adults. Conversely, no difference was observed between infected and noninfected children, but when they were stratified by age, an increased CD4+/CD28+ cell percentage was seen in the HP-positive group older than 10 years. The mean level of IgG anti-HP was lower in younger infected children, increased with age and correlated with CD4+ cells. Our data suggest that the immune response to HP infection vary according to the age. Low percentage of activated CD4+ cell may contribute to the lower level of serum IgG anti-HP observed in younger infected children. In addition, the CD4+ cell participation during the infection seems to begin after 10 years old, when the immune response becomes similar to that seen in adults.
Keywords: Antibodies, Bacterial/blood
Antigens, CD/analysis
B-Lymphocytes/classification
Helicobacter infections/immunology
Helicobacter pylori
Immunoglobulin G/blood
Issue Date: 2005
Publisher: Blackwell Publishing
Citation: SOARES, Taciana de Figueiredo et al. Phenotypic study of peripheral blood lymphocytes and humoral immune response in Helicobacter pylori infection according to age. Scand J Immunol., v. 62, n. 1, p. 63-70, July 2005.
DOI: 10.1111/j.1365-3083.2005.01638.x
ISSN: 0300-9475
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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