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https://www.arca.fiocruz.br/handle/icict/7380
A STUDY OF INTERLEUKIN 6 (IL-6) AND TUMOR NECROSIS FACTOR ALPHA (TNF-α) SERUM LEVELS IN RATS SUBJECTED TO FECAL PERITONITIS AND TREATED WITH INTRAPERITONEAL ROPIVACAINE
Author
Affilliation
Espirito Santo Federal University Department of Surgery. Division of Anesthesiology. Vitória, ES, Brazil.
EMESCAM. Vitória, ES, Brazil.
Universidade Federal de Minas Gerais. Surgical and Ophthalmological Applied Sciences Postgraduate Program. Belo Horizonte, MG, Brazil.
Espirito Santo Federal University. Department of Surgery. Division of Anesthesiology. Vitória, ES, Brazil.
Universidade Federal de Minas Gerais. Surgical and Ophthalmological Applied Sciences Postgraduate Program. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory analysis and Interpretation of data.
Minas Gerais Federal University. Department of Surgery. Belo Horizonte, MG, Brazil
Espirito Santo Federal University. Department of Surgery. Division of Anesthesiology. Vitória, ES, Brazil
EMESCAM. Vitória, ES, Brazil.
Universidade Federal de Minas Gerais. Surgical and Ophthalmological Applied Sciences Postgraduate Program. Belo Horizonte, MG, Brazil.
Espirito Santo Federal University. Department of Surgery. Division of Anesthesiology. Vitória, ES, Brazil.
Universidade Federal de Minas Gerais. Surgical and Ophthalmological Applied Sciences Postgraduate Program. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory analysis and Interpretation of data.
Minas Gerais Federal University. Department of Surgery. Belo Horizonte, MG, Brazil
Espirito Santo Federal University. Department of Surgery. Division of Anesthesiology. Vitória, ES, Brazil
Abstract
PURPOSE: The objective of this study was to assess the cytokine serum levels of IL-6 and TNF-α in rats subjected to fecal peritonitis and treated with peritoneal lavage with 0.2% ropivacaine by peritoneal lavage.
METHODS: We subjected 16 Wistar rats to laparotomy 6 hours after the induction of fecal peritonitis with autogenous stool and subsequently divided the rats randomly into 4 groups: I-control, no treatment; II- drying of the abdominal cavity; III- lavage of the abdominal cavity with 3 mL of 0.9% normal saline and drying; IV- lavage of the abdominal cavity with 3 mL of 0.2% ropivacaine and drying. Six hours following the laparotomy, the animals underwent cardiac puncture, and 1 mL of blood was collected for cytokine assessment before the animals were euthanized.
RESULTS: The lavage with ropivacaine resulted in smaller TNF-α levels compared with those observed in the other treatment groups (p <0.05). Regarding IL-6, the ropivacaine group showed lower cytokine levels than those observed in groups I and II, but there was no significant difference (p> 0.05) between groups III and IV.
CONCLUSION: Peritoneal lavage with 0.2% ropivacaine was shown to reduce plasma levels of IL-6 and TNF-α in the treatment of fecal peritonitis in rats
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