Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7439
Title: Revisiting proteus: do minor changes in lectin structure matter in biological activity? Lessons from and potential biotechnological uses of the Diocleinae subtribe lectins
Authors: Cavada, Benildo Sousa
Bessa, Theolis Costa Barbosa
Arruda, Sérgio Marcos
Grangeiro, Thalles Barbosa
Barral Netto, Manoel
Affilliation: Universidade Federal do Ceará. BioMol – Laboratório. Departamento de Bioquímica e Biologia Molecular. Fortaleza, CE, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imunoregulação. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imunoregulação. Salvador, BA, Brasil.
Universidade Federal do Ceará. BioMol – Laboratório. Departamento de Bioquímica e Biologia Molecular. Fortaleza, CE, Brasil / Universidade Federal do Ceará. Laboratório de Citogenética e Genética Molecular. Departamento de Biologia. Fortaleza, CE, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imunoregulação. Salvador, BA, Brasil.
Abstract: Significant differences in function have been observed among lectins structurally similar to concanavalin A, but their high homology with this widely used lectin has kept them in obscurity. The observation of large differences in the potency of many of these Diocleinae lectins as stimulators of Interferon-g production by human peripheral blood mononuclear cells has lead to a major effort to unravel their chemical structure and biological activity. Modeling studies of some of these lectins reveal conformational changes in side chains of some residues involved in the carbohydrate-binding site, with possible effects on the ability of these proteins to recognize specific carbohydrate structures. Additionally, all them constitute in fact a mixture of isolectins, which in different proportions could lead to diverse effects. The present review of the biological actions of Diocleinae lectins includes several in vitro and in vivo immunological findings, as well as their effects on insect growth and reproduction. In these systems Diocleinae lectins proved to be quite diverse in their potency. Such diversity in the biological activity of highly related proteins recalls the origin of the name protein: like Proteus, the capability of assuming various forms is the essential feature of this class of molecules.
DeCS: Lectinas/química
Lectinas/farmacologia
Sequência de Aminoácidos
Animais
Biotecnologia
Fabaceae/química
Fabaceae/genética
Humanos
Lectinas/genética
Dados de Sequência Molecular
Homologia de Sequência de Aminoácidos
Relação Estrutura-Atividade
Issue Date: 2001
Publisher: Bentham Science Publishers Ltd.
Citation: CAVADA, B. et al. Revisiting proteus: do minor changes in lectin structure matter in biological activity? Lessons from and potential biotechnological uses of the Diocleinae subtribe lectins. Current Protein and Peptide Science, v. 2, p. 123-135, 2001.
ISSN: 1389-2037
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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