Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7452
Title: In vivo protective effect of the lectin from Canavalia brasiliensis on BALB/c mice infected by Leishmania amazonensis
Authors: Barral Netto, Manoel
Von Sohsten, R. L
Teixeira, M
dosSantos, Washington Luis Conrado
Pompeu, Margarida Maria de Lima
Moreira, R. A
Oliveira, J. T
Cavada, Benildo Sousa
Falcoff, E
Barral, Aldina Maria Prado
Affilliation: Universidade Federal da Bahia. Hospital Univ. Prof. Edgard Santos. Serviço de lmunologia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / LAboratório Integrado de Microbiologia e Imunoregulação. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Univ. Prof. Edgard Santos. Serviço de lmunologia. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Univ. Prof. Edgard Santos. Serviço de lmunologia. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Univ. Prof. Edgard Santos. Serviço de lmunologia. Salvador, BA, Brasil
Universidade Federal do Ceará. Departamentos de Patologia & Bioquimica. Fortaleza, CE, Brasil
Universidade Federal do Ceará. Departamentos de Patologia & Bioquimica. Fortaleza, CE, Brasil
Universidade Federal do Ceará. Departamentos de Patologia & Bioquimica. Fortaleza, CE, Brasil
Universidade Federal do Ceará. Departamentos de Patologia & Bioquimica. Fortaleza, CE, Brasil
lnstitut Curie. Unitk INSERM. PAris, France
Universidade Federal da Bahia. Hospital Univ. Prof. Edgard Santos. Serviço de lmunologia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Abstract: In vivo administration of Canavalia brasiliensis lectin (at the time of infection, or maintained throughout the infection) reduced the lesions of highly susceptible BALB/c mice infected by Leishmania amazonensis. At the doses used C. brasiliensis lectin (ConBr) does not interfere with penetration or fate of Leishmania in the macrophages in vitro. Since Interferon-gamma (IFN-gamma) is the major macrophage activating factor, and considered a critical element in the successful immune response against leishmaniasis, we explored its participation in this phenomenon. ConBr either in vivo or in vitro induced IFN-gamma production in normal or in leishmania-infected BALB/c mice. However we were unable to change the course of disease by in vivo IFN-gamma administration (although IFN-gamma preparations were effective in inducing a leishmanicidal effect in vitro on L. amazonensis-infected peritoneal macrophages). Additionally, IFN-gamma neutralization with anti-IFN-gamma monoclonal antibody did not alter the protection conferred by ConBr administration. These data show that lectin administration in vivo is protective in the otherwise unchecked L. amazonensis infection of BALB/c mice, and suggest that such effect is not mediated by IFN-gamma.
Keywords: Leishmaniasis
Leishmania
Tegumentary leishmaniasis
Experimental leishmaniasis
Canavalia brasiliensis
Interferon-y
Animais
Feminino
DeCS: Interferon gama/biossíntese
Lectinas/uso terapêutico
Leishmania mexicana
Leishmaniose Cutânea/terapia
Interferon gama/farmacocinética
Leishmaniose Cutânea/imunologia
Leishmaniose Cutânea/parasitologia
Macrófagos Peritoneais/efeitos de drogas
Macrófagos Peritoneais/parasitologia
Camundongos
Camundongos Endogâmicos BALB C
Ratos
Issue Date: 1996
Publisher: Elsevier Science B.V
Citation: BARRAL-NETTO, M. et al. In vivo protective effect of the lectin from Canavalia brasiliensis on BALB/c mice infected by Leishmania amazonensis. Acta Tropica, v. 60, n. 4, p. 237-250 237, 1996.
ISSN: 0001-706X
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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