Please use this identifier to cite or link to this item:
https://www.arca.fiocruz.br/handle/icict/7493
HCV GENOTYPE DETERMINATION IN MONOINFECTED AND HIV CO-INFECTED PATIENTS IN CUBA.
HIV
HCV/HIV co-infection
Molecular epidemiology
Phylogenetic analysis
Risk groups
RNA Viral/genética
Infecções por HIV/virologia
Hepacivirus/genética
Hepatite C/virologia
Adolescente
Adulto
Idoso
Criança
Coinfecção/epidemiologia
Cuba/epidemiologia
Primers do DNA/genética
Feminino
Genótipo
Infecções por HIV/epidemiologia
Hepacivirus/classificação
Hepatite C/diagnóstico
Hepatite C/epidemiologia
Homossexualidade Masculina
Humanos
Masculino
Meia-Idade
Epidemiologia Molecular
10.1016/j.trstmh.2012.08.001
Author
Rodríguez Lay, Licel de los Angeles
Villalba, Maria Caridad Montalvo
Corredora, Marité Bello
Frómeta, Susel Sariego
Hernández, Jeny Marante
Carrera, Santiago Dueñas
Wonga, Meilin Sánchez
Samada, Marcia
Núñez, Milay Bello
Alonso, Lidunka Valdes
Silva Filho, Hermes Pedreira da
Hübschenf, Judith M.
Reis, Mitermayer Galvão dos
Villalba, Maria Caridad Montalvo
Corredora, Marité Bello
Frómeta, Susel Sariego
Hernández, Jeny Marante
Carrera, Santiago Dueñas
Wonga, Meilin Sánchez
Samada, Marcia
Núñez, Milay Bello
Alonso, Lidunka Valdes
Silva Filho, Hermes Pedreira da
Hübschenf, Judith M.
Reis, Mitermayer Galvão dos
Affilliation
Center for Genetic Engineering and Biotechnology (CIGB). Havana, Cuba
Institute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, Cuba
Institute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, Cuba
Institute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, Cuba
Center for Genetic Engineering and Biotechnology (CIGB). Havana, Cuba
Center for Genetic Engineering and Biotechnology (CIGB). Havana, Cuba
Center for Genetic Engineering and Biotechnology (CIGB). Havana, Cuba
Centro de Investigaciones Médico-Quirúrgicas. CIMEQ. Havana, Cuba
Ivan Portuondo General Hospital. San Antonio de los Baños. Havana, Cuba
Institute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, Cuba
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Institute of Immunology. CRP-Santé/Laboratoire National de Santé. Luxembourg, Luxembourg
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Institute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, Cuba
Institute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, Cuba
Institute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, Cuba
Center for Genetic Engineering and Biotechnology (CIGB). Havana, Cuba
Center for Genetic Engineering and Biotechnology (CIGB). Havana, Cuba
Center for Genetic Engineering and Biotechnology (CIGB). Havana, Cuba
Centro de Investigaciones Médico-Quirúrgicas. CIMEQ. Havana, Cuba
Ivan Portuondo General Hospital. San Antonio de los Baños. Havana, Cuba
Institute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, Cuba
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Institute of Immunology. CRP-Santé/Laboratoire National de Santé. Luxembourg, Luxembourg
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Abstract
With the aim to characterize the HCV genotype distribution in Cuba, sera were collected
from two subgroups: HCV-monoinfected and HCV/HIV co-infected patients. A combination
of reverse transcription-PCR using genotype-specific primers, restriction fragment
length polymorphism and sequencing was used to determine the genotype of 84 samples.
Seventy-nine (94%) showed single infections (10 [12%] were genotype 1a and 69 [82%]
genotype 1b) and 5 (6%) samples corresponded to mixed infections (2 [2%] with genotypes
1a/3a and 1 sample [1%] each with 1b/3a, 1b/4a and 1a/1b/3a). HCV/HIV co-infected
subjects had a higher frequency of mixed infections (p=0.08), infection with genotype 3a
(p=0.18) and for the first time genotype 4a was found. There was no association of any demographic
characteristics with any specific genotype although HCV/HIV co-infected patients
showed a tendency to have mixed genotypes in those older than 45 years of age (p=0.11).
Phylogenetic analysis showed that HCV isolates clustered with subtypes 1b (n=15, maximal
genetic distance 2.51%) and 1a (n=2, maximal genetic distance 0.35%). This report presents
the prevalence of HCV genotypes in monoinfected and HIV co-infected patients, mixed HCV
infections in HCV/HIV co-infected men who have sex with men with high-risk sexual practices
and for the first time identifies that the uncommon genotype 4a can be present in a
patient co-infected with HIV.
Keywords
HCVHIV
HCV/HIV co-infection
Molecular epidemiology
Phylogenetic analysis
Risk groups
RNA Viral/genética
DeCS
Coinfecção/virologiaInfecções por HIV/virologia
Hepacivirus/genética
Hepatite C/virologia
Adolescente
Adulto
Idoso
Criança
Coinfecção/epidemiologia
Cuba/epidemiologia
Primers do DNA/genética
Feminino
Genótipo
Infecções por HIV/epidemiologia
Hepacivirus/classificação
Hepatite C/diagnóstico
Hepatite C/epidemiologia
Homossexualidade Masculina
Humanos
Masculino
Meia-Idade
Epidemiologia Molecular
Publisher
Elsevier Ltd
Citation
RODRIGUEZ LAY, L. de los A. et al. HCV genotype determination in monoinfected and HIV co-infected patients in Cuba. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 106, n. 12, p. 711–717, 2012.ISSN
1878-350310.1016/j.trstmh.2012.08.001
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