Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7549
Title: Prevalence of hepatitis C virus (HCV) infection and HCV genotypes of hemodialysis patients in Salvador, Northeastern Brazil
Authors: Silva, Luciano Kalabric
Silva, M. B. S.
Rodart, Itatiana Ferreira
Lopes, Gisele Barreto
Costa, F. Q.
Melo, M. E.
Gusmão, E.
Reis, Mitermayer Galvão dos
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil
Universidade Federal da Bahia. Faculdade de Medicina. Departamento de Medicina Interna. Salvador, BA, Brasil
4Comissão Estadual de Nefrologia. Salvador, BA, Brasil
Abstract: Hepatitis C virus (HCV) infection has been identified as the major cause of chronic liver disease among patients on chronic hemodialysis (HD), despite the important reduction in risks obtained by testing candidate blood donors for anti-HCV antibodies and the use of recombinant erythropoietin to treat anemia. A cross-sectional study was performed to estimate the prevalence of HCV infection and genotypes among HD patients in Salvador, Northeastern Brazil. Anti- HCV seroprevalence was determined by ELISA in 1243 HD patients from all ten different dialysis centers of the city. HCV infection was confirmed by RT-PCR and genotyping was performed by restriction fragment length polymorphism. Anti-HCV seroprevalence among HD patients was 10.5% (95% CI: 8.8-12.3) (Murex anti-HCV, Abbott Murex, Chicago, IL, USA). Blood samples for qualitative HCV detection and genotyping were collected from 125/130 seropositive HD patients (96.2%). HCV-RNA was detected in 92/125 (73.6%) of the anti-HCV-positive patients. HCV genotype 1 (77.9%) was the most prevalent, followed by genotype 3 (10.5%) and genotype 2 (4.6%). Mixed infections of genotypes 1 and 3 were found in 7.0% of the total number of patients. The present results indicate a significant decrease in anti-HCV prevalence from 23.8% detected in a study carried out in 1994 to 10.5% in the present study. The HCV genotype distribution was closely similar to that observed in other hemodialysis populations in Brazil, in local candidate blood donors and in other groups at risk of transfusion-transmitted infection.
Keywords: Hepatitis C
Seroprevalence
Viremia
Hemodialysis
Genotype
Brazil
DeCS: Genótipo
Hepacivirus/genética
Anticorpos Anti-Hepatite C/sangue
Hepatite C/epidemiologia
Diálise Renal/efeitos adversos
Brasil/epidemiologia
Estudos Transversais
Eletroforese em Gel de Ágar
Ensaio de Imunoadsorção Enzimática
Hepacivirus/imunologia
Hepatite C/diagnóstico
Hepatite C/etiologia
Humanos
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Prevalência
RNA Viral/análise
Estudos Soroepidemiológicos
Issue Date: 2006
Publisher: Faculdade de Medicina de Ribeirao Preto Associacao Brasileira de Divulgacao
Citation: SILVA, L. K. et al. Prevalence of hepatitis C virus (HCV) infection and HCV genotypes of hemodialysis patients in Salvador, Northeastern Brazil. Brazilian Journal of Medical and Biological Research, v. 39, n. 5, p. 595-602, 2006.
ISSN: 0100-879X
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Silva LK Prevalence of hepatitis....pdf577.31 kBAdobe PDFThumbnail
View/Open



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.