Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7728
Title: Leishmania amazonensis fails to induce the release of reactive oxygen intermediates by CBA macrophages.
Authors: Almeida, Taís Fontoura de
Palma, Luana Carneiro
Mendez, Lucas C
Dutra, Alberto Augusto Noronha
Veras, Patrícia Sampaio Tavares
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
Abstract: CBA mouse macrophages effectively control Leishmania major infection, yet are permissive to Leishmania amazonensis. It has been established that some Leishmania species are destroyed by reactive oxygen species (ROS). However, other species of Leishmania exhibit resistance to ROS or even down-modulate ROS production. We hypothesized that L. amazonensis-infected macrophages reduce ROS production soon after parasite-cell interaction. Employing a highly sensitive analysis technique based on chemiluminescence, the production of superoxide (O(·-)(2)) and hydrogen peroxide (H(2)O(2)) by L. major- or L. amazonensis-infected CBA macrophages were measured. L. major induces macrophages to release levels of (O(·-)(2)) 3·5 times higher than in uninfected cells. This (O(·-)(2)) production is partially dependent on NADPH oxidase (NOX) type 2. The level of accumulated H(2)O(2) is 20 times higher in L. major-than in L. amazonensis-infected cells. Furthermore, macrophages stimulated with L. amazonensis release amounts of ROS similar to uninfected cells. These findings support previous studies showing that CBA macrophages are effective in controlling L. major infection by a mechanism dependent on both (O(·-)(2)) production and H(2)O(2) generation. Furthermore, these data reinforce the notion that L. amazonensis survive inside CBA macrophages by reducing ROS production during the phagocytic process.
Keywords: Leishmania
Macrophage
Reactive oxygen intermediates
DeCS: Leishmania mexicana/imunologia
Macrófagos/imunologia
Macrófagos/parasitologia
Espécies de Oxigênio Reativas/metabolismo
Animais
Peróxido de Hidrogênio/análise
Leishmania/imunologia
Leishmania major/imunologia
Medições Luminescentes
Camundongos
Camundongos Endogâmicos CBA
NADPH Oxidase/metabolismo
Superóxidos/análise
Issue Date: 2012
Publisher: Blackwell Publishing Ltd
Citation: ALMEIDA, T. F. et al. Leishmania amazonensis fails to induce the release of reactive oxygen intermediates by CBA macrophages. Parasite Immunology, v. 34, n. 10, p. 492-498, 2012.
DOI: 10.1111/j.1365-3024.2012.01384.x
ISSN: 1365-3024
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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