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MOLECULAR CHARACTERIZATION OF THE HUMAN T CELL LYMPHOTROPIC VIRUS TYPE 2 LONG TERMINAL REPEAT REGION: A DISCUSSION ABOUT POSSIBLE INFLUENCES AT VIRAL GENE EXPRESSION
HTLV-2
Brasil
Reação em cadeia da polimerase
PCR
Filogenia
Polimorfismo
Author
Affilliation
Abstract
This study aimed to identify nucleotide signatures in the promoter region of human T cell lymphotropic virus
type 2 (HTLV-2) isolated from infected individuals from Salvador, Brazil and in sequences from the GenBank
database. DNA samples from HTLV-2-infected individuals were submitted to nested polymerase chain reaction
(PCR) and sequencing, and molecular analyses were performed using bioinformatics tools. The phylogeny of
HTLV-2 strains isolated from patients from Salvador reveals that all sequences were subtype c. One hundred
and fifty-one sequences from GenBank were selected, among which 30 belong to subtype a, 88 to subtype b, 32 to
subtype c, and one to subtype d. Subtype-specific signatures were identified as well as mutations resulting in
loss or gain of motifs important to transcription regulation. The subtypes a and b have two E box motifs, while
subtypes c and d have only one. These polymorphisms may impact viral fitness and infection outcome and
should be more closely investigated.
Keywords in Portuguese
Virus linfotropico T humano tipo 2HTLV-2
Brasil
Reação em cadeia da polimerase
PCR
Filogenia
Polimorfismo
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