Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8192
Title: Molecular epidemiology of HIV-1 in Brazil: high prevalence of HIV-1 subtype B and identification of an HIV-1 subtype D infection in the city of Rio de Janeiro, Brazil. Evandro Chagas Hospital AIDS Clinical Research Group.
Authors: Morgado, Mariza Gonçalves
Guimarães, Monick Lindenmeyer
Gripp, Carmen B. G
Costa, Catia Ines
Neves Junior, Ivan
Veloso, Valdiléia Gonçalves
Carvalho, Maria Inês Linhares de
Castello-Branco, Luis Roberto Ribeiro
Bastos, Francisco Inácio Pinkusfeld Monteiro
Kuiken C, Carla
Castilho, Euclides Ayres de
Castro Filho, Bernardo Galvão
Bongertz, Vera
Evandro Chagas Hospital AIDS Clinical Research Group
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil
Banco da Previdência Outpatient clinic.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil / Banco da Previdência Outpatient clinic.
Fundação Oswaldo Cruz. CICT. Department of Health Information. Rio de Janeiro, RJ, Brasil
Los Alamos National Laboratories. Los Alamos, New Mexico, USA
Fundação Oswaldo Cruz. CICT. Department of Health Information. Rio de Janeiro, RJ, Brasil / Brazilian Ministry of Health. National Coordination of STD/AIDS. Brasília, DF, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil
Abstract: HIV-1-positive individuals were recruited from January 1993 to December 1996 from several cohorts receiving follow-up in the city of Rio de Janeiro, Brazil, to evaluate HIV-1 genetic variability and the potential association with modes of transmission. HIV-1 subtyping was carried out using the heteroduplex mobility assay (HMA), and those samples corresponding to the typical Brazilian subtype B variant were further identified based on the Fok I restriction fragment length polymorphism (RFLP). DNA sequencing was performed to evaluate one case of subtype D infection. From the 131 HIV-1-positive individuals analyzed, 106 (80.9%) could be identified as infected by subtype B and 20 (15.3%) by subtype F. One of the samples (0.8%) was classified as subtype D. DNA samples from 4 patients (3.0%) did not yield polymerase chain reaction (PCR)-amplified products to be typed. Based on the Fok I RFLP, 39 of the 106 subtype B samples (37%) were identified as corresponding to the typical Brazilian subtype B variant containing the GWGR motif at the tip of the V3 loop. No statistically significant association could be detected between HIV-I subtypes and modes of transmission, exposure categories, or gender. This is the first reported case of HIV-1 subtype D infection in Brazil
Keywords: HIV-1 subtypes in Brazil
Heteroduplex mobility assay
HIV-1 subtype D infection
DeCS: Infecções por HIV/epidemiologia
HIV-1/classificação
Sequência de Aminoácidos
Brasil/epidemiologia
Estudos de Coortes
Sequência Consenso
DNA Viral/química
Feminino
Produtos do Gene env/química
Variação Genética
Infecções por HIV/transmissão
HIV-1/genética
Humanos
Masculino
Epidemiologia Molecular
Dados de Sequência Molecular
Ácidos Nucleicos Heteroduplexes
Filogenia
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Prevalência
Alinhamento de Sequência
Distribuição por Sexo
População Urbana
Issue Date: 1998
Publisher: Lippincott-Raven Publishers, Philadelphia
Citation: MORGADO, M. G. et al. Molecular epidemiology of HIV-1 in Brazil: high prevalence of HIV-1 subtype B and identification of an HIV-1 subtype D infection in the city of Rio de Janeiro, Brazil. Evandro Chagas Hospital AIDS Clinical Research Group. Journal of Acquired Immune Deficience and Syndromes Human Retrovirology, v. 18, n. 5, p. 488-494, 1998.
ISSN: 1077-9450
Copyright: open access
Appears in Collections:INI - Artigos de Periódicos
BA - IGM - Artigos de Periódicos

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