Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8342
Title: A glass wool-based method for purifying Trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptide
Authors: Pinho, Rosa Teixeira de
Dutra, Helio dos Santos
De Simone, Salvatore Giovanni
Pontes-de-Carvalho, Lain Carlos
Affilliation: WHO Collaborating Center on Immunology of Parasitic Diseases. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.
WHO Collaborating Center on Immunology of Parasitic Diseases. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Biologia Celular e Molecular. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Niteroi, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.
Abstract: Glass wool, hydrophilic cotton wool, non-electrically charged BIO-GEL P2 and common tissue paper columns were used to purify trypomastigotes from a mixed Trypanosoma cruzi population grown in axenic culture medium. With all these columns, highly purified (up to 98%) trypomastigote preparations were obtained. Trypomastigote yields from cotton wool, BIO-GEL P2 and common tissue paper columns were not as high as from glass wool columns, from which yields varied from 69 to 80%. Purification on glass wool did not affect trypomastigote infectivity or virulence. Dead trypomastigotes could not be purified on glass wool columns. A glass-adherent amphiphilic peptide of 45 kDa, present in the cell membrane, was isolated from epimastigote but not from trypomastigote preparations.
Keywords: Trypanosoma cruzi
Glass wool purification
Trypomastigotes
Surface peptide
Triton X-114
DeCS: Doença de Chagas/parasitologia
Vidro
Proteínas de Membrana/análise
Proteínas de Protozoários/análise
Trypanosoma cruzi/isolamento & purificação
Animais
Cromatografia DEAE-Celulose
Eletroforese em Gel de Poliacrilamida
Insetos Vetores/parasitologia
Masculino
Proteínas de Membrana/metabolismo
Camundongos
Proteínas de Protozoários/metabolismo
Triatoma/parasitologia
Trypanosoma cruzi/química
Issue Date: 1991
Publisher: Elsevier Science Publishers B.V
Citation: PINHO, Rosa Teixeira de et al. A glass wool-based method for purifying Trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptide. Acta Tropica, v. 50, n. 1, p. 29-38, 1991.
ISSN: 0001-706X
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos
BA - IGM - Artigos de Periódicos

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