Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8386
Title: Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis
Authors: Gonçalves, Thais Oliveira
Costa, Dirceu Joaquim
Brodskyn, Claudia Ida
Duarte, Poliana Mendes
César Neto, João Batista
Nogueira Filho, Getulio
Affilliation: UNICAMP. Faculty of Dentistry of Piracicaba. Department of Prosthodontics and Periodontics. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imunologia e Parasitologia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imunologia e Parasitologia. Salvador, BA, Brasil
Guarulhos University. Department of Periodontics, Dental Research Division. Guarulhos, São Paulo, Brasil
University of São Paulo. School of Dentistry. USP. Department of Periodontology. São Paulo, SP, Brasil
University of Manitoba. Faculty of Dentistry. Department of Dental Diagnostic and Surgical Sciences. Division of Periodontics. Bannatyne Ave, Winnipeg, Canada
Abstract: Objective: The emergence of periodontal medicine increased interest in defining the behaviour of peripheral blood cells in periodontitis subjects in comparison with healthy group. The aim of this study was to evaluate the levels of interleukin (IL)-8, tumour necrosis factora (TNF-a), IL-6 and IL-10 released by Escherichia coli lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMC) obtained from the peripheral blood of chronic periodontitis subjects. Design: PBMC samples were isolated from 19 systemically healthy donors, divided into generalized chronic periodontitis (n = 10) and healthy (n = 9) subjects. Cells were incubated for 24–48 h in 500 mL wells containing RPMI 1640 and stimulated with 1.0 ng/mL of E. coli LPS. Supernatants were used to quantify the amounts of IL-8, TNF-a, IL-6 and IL-10 released using enzyme-linked immunosorbent assay (ELISA). Results: PBMC cells from periodontitis subjects released higher levels of TNF-a and IL-6 than those from healthy subjects (P < 0.05). Conversely, the supernatants of the stimulated PBMC cells obtained from healthy subjects presented higher amounts of IL-8 than those from periodontitis (P < 0.05). No differences were observed in the levels of IL-10 (P > 0.05) between groups. Conclusion: In conclusion, the results of the present study showed that E. coli LPS-stimulated PBMC from subjects with periodontitis present a different pattern of cytokine release when compared to PBMC from healthy subjects. This phenomenon could have implications locally, in periodontitis, as well as in systemic diseases.
Keywords: Periodontitis
Cytokines
ELISA
Mononuclear cell
PBMC
DeCS: Periodontite Crônica/sangue
Citocinas/sangue
Leucócitos Mononucleares/metabolismo
Adulto
Idoso
Células Cultivadas
Escherichia coli
Feminino
Hemorragia Gengival/sangue
Humanos
Mediadores da Inflamação/sangue
Interleucina-10/sangue
Leucócitos Mononucleares/efeitos de drogas
Lipopolissacarídeos/farmacologia
Masculino
Meia-Idade
Perda da Inserção Periodontal/sangue
Bolsa Periodontal/sangue
Hábito de Fumar
Fator de Necrose Tumoral alfa/sangue
Issue Date: 2010
Publisher: Elsevier Ltd
Citation: GONÇALVES, T. O. et al. Release of cytokines by stimulated peripheral blood mononuclear cells in chronic periodontitis. Archives of Oral Biology, v. 55, n. 12, p. 975-980, 2010.
DOI: 10.1016/j.archoralbio.2010.08.002
ISSN: 1879-1506
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Gonçalves TO Release of cytokines.....pdf164 kBAdobe PDFView/Open



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.