| Author | Martins, Heliana Figueiredo | |
| Author | Pinto, Douglas Pereira | |
| Author | Nascimento, Viviane de Assis | |
| Author | Marques, Marlice Aparecida Sipoli | |
| Author | Amendoeira, Fábio Coelho | |
| Access date | 2014-10-05T19:49:57Z | |
| Available date | 2014-10-05T19:49:57Z | |
| Document date | 2013 | |
| Citation | MARTINS, H. F. et al. Determination of levodopa in human plasma by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS): application to a bioequivalence study. Química Nova, São Paulo, v. 36, n. 1, p. 171-176. 2013. | pt_BR |
| ISSN | 0100-4042 | |
| URI | https://www.arca.fiocruz.br/handle/icict/8506 | |
| Language | eng | pt_BR |
| Publisher | Sociedade Brasileira de Química | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Levodopa | pt_BR |
| Subject in Portuguese | HPLC-ESI-MS/MS | pt_BR |
| Subject in Portuguese | Bioequivalência | pt_BR |
| Title | Determination of levodopa in human plasma by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS): application to a bioequivalence study | en |
| Type | Article | pt_BR |
| DOI | http://dx.doi.org/10.1590/S0100-40422013000100028 | |
| Abstract | A sensitive, accurate and simple method using HPLC-MS/MS was developed and validated for levodopa quantitation in human plasma. Analysis was achieved on a pursuit® C18 analytical column (5 µm; 150 x 4.6 mm i.d.) using a mobile phase (methanol and water , 90:10, v/v) containing formic acid 0.5% v/v, after extracting the samples using a simple protein plasma precipitation with perchloric acid. The developed method was validated in accordance with ANVISA guidelines and was successfully applied to a bioequivalence study in 60 healthy volunteers demonstrating the feasibility and reliability of the proposed method. | en |
| Affilliation | Fundação Oswaldo Cruz. Laboratório de Farmacocinética. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Laboratório de Farmacocinética. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Laboratório de Farmacocinética. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Química Analítica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Farmacologia e Toxicologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Levodopa | en |
| Subject | HPLC-ESI-MS/MS | en |
| Subject | Bioequivalence | en |
| DeCS | Levodopa | pt_BR |
| DeCS | Cromatografia Líquida de Alta Pressão | pt_BR |
| DeCS | Equivalência Terapêutica | pt_BR |
