Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8907
Title: Influence of the HPA axis on the inflammatory response in cutaneous wounds with the use of 670-nm laser photobiomodulation.
Authors: Vilela, Deyla Duarte Carneiro
Chamusca, Flávia Villela
Andrade, Jorge Clarêncio de Souza
Vallve, Maria de Lourdes Farre
Gonzalez, Ana Cristina
Andrade, Zilton de Araújo
Medrado, Alena Ribeiro Alves Peixoto
Reis, Sílvia Regina de Almeida
Affilliation: Escola Bahiana de Medicina e Saúde Pública. Dentistry Course. Salvador, BA, Brasil
Escola Bahiana de Medicina e Saúde Pública. Dentistry Course. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, Brasil
Escola Bahiana de Medicina e Saúde Pública. Dentistry Course. Salvador, BA, Brasil
Escola Bahiana de Medicina e Saúde Pública. Dentistry Course. Salvador, BA, Brasil
Abstract: This study evaluated the influence of hypothalamic–pituitary–adrenal (HPA) axis in cutaneous wounds subjected to laser biomodulation. A total of 48 rats were divided into two groups: Group I (GI) with 24 adrenalectomized animals and Group II (GII) with 24 non-adrenalectomized animals. Each group was divided into two subgroups: the irradiated subgroup which laser was applied to four points at the edges of the wound (670 nm laser, 9 mW) and control subgroup. Rats in each subgroup were sacrificed at 24 or 72 h. Adrenal glands were only removed from GI rats. Three days after adrenalectomy, a cutaneous wound was made. An immunohistochemical analysis was performed using anti-CD45 and anti-CD8 antibodies. Flow cytometry was used to count T lymphocytes and their subpopulations in blood. Decreases in the number of CD45-positive inflammatory cells and in the total numbers of CD8- and CD45-positive cells were observed in histological sections of adrenalectomized animals subjected to laser biomodulation at 24 h. Similar results were observed for distribution of total lymphocytes in blood (p < 0.05). The action of 670 nm laser does not depend exclusively on HPA axis. It is believed that corticosteroid-promoting enzymes liberated in non-adrenal tissues may influence immune response under the influence of this type of phototherapy.
Keywords: Low-intensity laser therapy
CD8
CD45
CD4
T lymphocytes
DeCS: Glândulas Suprarrenais/fisiopatologia
Sistema Hipotálamo-Hipofisário/fisiopatologia
Terapia a Laser
Pele/lesões
Pele/fisiopatologia
Glândulas Suprarrenais/cirurgia
Adrenalectomia/efeitos adversos
Animais
Linfócitos T CD4-Positivos/citologia
Linfócitos T CD8-Positivos/citologia
Inflamação/sangue
Inflamação/imunologia
Inflamação/cirurgia
Masculino
Ratos
Ratos Wistar
Pele/imunologia
Issue Date: 2012
Publisher: Elsevier
Citation: VILELA, D. D. C. et al. Influence of the HPA axis on the inflammatory response in cutaneous wounds with the use of 670-nm laser photobiomodulation. Journal of Photochemistry and Photobiology: Biology, v. 116, p. 114-120, 2012.
DOI: dx.doi.org/10.1016/j.jphotobiol.2012.05.018
ISSN: 1873-2682
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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