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Title: Participation of cytokines in the necrotic-inflammatory lesions in the heart and skeletal muscles of Calomys callosus infected with Trypanosoma cruzi
Authors: Santos, Isis Fernandes Magalhães
Andrade, Sonia Gumes
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Abstract: Calomys callosus, a sylvatic reservoir of Trypanosoma cruzi, when infected with the Colombian strain (Biodeme Type III, T. cruzi I ) develops necrotic-inflammatory lesions and intense early fibrogenesis in the heart and skeletal muscles, that spontaneously regress. Participation of pro-inflammatory and pro-fibrogenic cytokines, such as tumor necrosis factor-alpha (TNF-α), gamma interferon (IFN-γ) , and tumor growth factor-beta (TGF-β), in the pathogenesis of the lesions is herein studied. Eighty C. callosus weighing 20 to 30 g were used. Seventy of them were inoculated with the Colombian strain (105 blood forms) and 10 were maintained as intact non-infected controls. After infection, C. callosus were sacrificed at different time-points from 15 to 70 days. The heart and skeletal muscle were processed for histopathology and cryopreserved for immunohistochemistry. Early necrotic lesions of parasitized skeletal muscle and myocardium with intense inflammatory lesions were present. Search for the in situ presence of TNF-α and IFN-γ, was performed using rat-IgG anti-mouse antibodies against these cytokines. For the in situ search of TGF-β, rabbit IgG anti-mouse antibodies were used. Immunolabeling of the cytokines in tissues of infected C. callosus was successful. The cytokines TNF-α, IFN-γ , and TGF-β were detected in the cytoplasm of macrophages and in the necrotic material from 15 to 45 days post-infection, decreasing their intensity until complete disappearance by the 65th day, which correlated with subsiding histopathological lesions. These findings suggest the participation of these cytokines in the control of parasite multiplication, in the development of an early fibrogenesis and in the regression of fibrotic-inflammatory lesions observed in C. callosus.
Keywords: Trypanosoma cruzi
Calomys callosus
Tumor necrosis factor-alpha
Gamma interferon
Tumor growth factor-beta
DeCS: Doença de Chagas
Músculo Esquelético
Trypanosoma cruzi
Fator Transformador de Crescimento beta
Fator de Necrose Tumoral alfa
Interferon gama
Issue Date: 2005
Citation: MAGALHÃES-SANTOS, I. F.; ANDRADE, S. G. Clinics in Sports Medicine. Memorias do Instituto Oswaldo Cruz, Rio de Janeiro, v.100, n. 5, p. 555-561. aug., 2005
ISSN: 0074-0276
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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