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Title: Development of a non-destructive method for determining protein nitrogen in a yellow fever vaccine by near infrared spectroscopy and multivariate calibration
Authors: Dabkiewicz, Vanessa Emídio
Abrantes, Shirley de Mello Pereira
Cassella, Ricardo Jorgensen
Affilliation: Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Departamento de Qualidade. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Química. Rio de Janeiro, RJ, Brasil.
Fluminense Federal University. Department of Analytical Chemistry. Niterói, RJ, Brazil.
Abstract: Near infrared spectroscopy (NIR) with diffuse reflectance associated to multivariate calibration has as main advantage the replacement of the physical separation of interferents by the mathematical separation of their signals, rapidly with no need for reagent consumption, chemical waste production or sample manipulation. Seeking to optimize quality control analyses, this spectroscopic analytical method was shown to be a viable alternative to the classical Kjeldahl method for the determination of protein nitrogen in yellow fever vaccine. The most suitable multivariate calibration was achieved by the partial least squares method (PLS) with multiplicative signal correction (MSC) treatment and data mean centering (MC), using a minimum number of latent variables (LV) equal to 1, with the lower value of the square root of the mean squared prediction error (0.00330) associated with the highest percentage value (91%) of samples. Accuracy ranged 95 to 105% recovery in the 4000–5184 cm−1 region.
Keywords: Nitrogen
Near infrared
Multivariate calibration
Cluster analysis
Mahalanobis distance
Issue Date: 2018
Publisher: Elsevier
Citation: DABKIEWICZ, Vanessa Emídio; ABRANTES, Shirley de Mello Pereira; CASSELLA, Ricardo Jorgensen et al. Development of a non-destructive method for determining protein nitrogen in a yellow fever vaccine by near infrared spectroscopy and multivariate calibration. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, v. 201, p. 170–177, 2018.
DOI: 10.1016/j.saa.2018.04.042
ISSN: 1386-1425
Copyright: restricted access
Appears in Collections:INCQS - Artigos de Periódicos
Biomanguinhos - Artigos de Periódicos
INI - Artigos de Periódicos

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